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Literature summary extracted from

  • Feth, F.; Arfmann, H.A.; Wray, V.; Wagner, K.G.
    Determination of putrescine N-methyltransferase by high performance liquid chromatography (1985), Phytochemistry, 24, 921-923.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.53 0.15
-
 S-adenosyl-L-methionine
-
 Datura stramonium
2.1.1.53 0.88
-
 putrescine
-
 Datura stramonium

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.53 Datura stramonium
-
-
-
2.1.1.53 Nicotiana tabacum
-
 var. Samsun
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.53
-
 Nicotiana tabacum
2.1.1.53
-
 Datura stramonium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.53 root
-
 Nicotiana tabacum
-
2.1.1.53 root
-
 Datura stramonium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.53 30
-
 assay at Nicotiana tabacum
2.1.1.53 30
-
 assay at Datura stramonium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.53 4
-
 3 h: 75% loss of activity, presence of 12.5% w/v glucose: 35% loss of activity Nicotiana tabacum
2.1.1.53 4
-
 3 h: 75% loss of activity, presence of 12.5% w/v glucose: 35% loss of activity Datura stramonium