BRENDA - Enzyme Database

Partitioning roles of side chains in affinity, orientation, and catalysis with structures for mutant complexes: Asparagine-229 in thymidylate synthase

Finer-Moore, J.S.; Liu, L.; Schafmeister, C.E.; Birdsall, D.L.; Mau, T.; Santi, D.V.; Stroud, R.M.; Biochemistry 35, 5125-5136 (1996)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.1.1.45
-
Lactobacillus casei
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.45
0.005
-
2'-deoxyuridylate
wild-type
Lactobacillus casei
2.1.1.45
0.01
-
methylenetetrahydrofolate
wild-type
Lactobacillus casei
2.1.1.45
0.017
-
2'-deoxyuridylate
mutant TS N229C
Lactobacillus casei
2.1.1.45
0.05
-
2'-deoxyuridylate
mutant TS N229D
Lactobacillus casei
2.1.1.45
0.092
-
methylenetetrahydrofolate
mutant TS N229C; mutant TS N229D
Lactobacillus casei
2.1.1.45
0.15
-
2'-deoxycytidine 5'-monophosphate
mutant TS N229D
Lactobacillus casei
2.1.1.45
0.17
-
methylenetetrahydrofolate
mutant TS N229D, dCMP as substrate
Lactobacillus casei
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Lactobacillus casei
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Lactobacillus casei TS N229D
-
dihydrofolate + dTMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.45
Lactobacillus casei
-
and strain TS N229C
-
2.1.1.45
Lactobacillus casei TS N229D
-
and strain TS N229C
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate
only mutant TS N229D
485410
Lactobacillus casei
dihydrofolate + cytidine phosphate
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate
only mutant TS N229D
485410
Lactobacillus casei TS N229D
dihydrofolate + cytidine phosphate
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei TS N229D
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei TS N229D
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
additional information
dCMP is no substrate, exception mutant TS N229D
485410
Lactobacillus casei
?
-
-
-
-
2.1.1.45
additional information
dCMP is no substrate, exception mutant TS N229D
485410
Lactobacillus casei TS N229D
?
-
-
-
-
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.1.1.45
-
Lactobacillus casei
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.45
0.005
-
2'-deoxyuridylate
wild-type
Lactobacillus casei
2.1.1.45
0.01
-
methylenetetrahydrofolate
wild-type
Lactobacillus casei
2.1.1.45
0.017
-
2'-deoxyuridylate
mutant TS N229C
Lactobacillus casei
2.1.1.45
0.05
-
2'-deoxyuridylate
mutant TS N229D
Lactobacillus casei
2.1.1.45
0.092
-
methylenetetrahydrofolate
mutant TS N229C; mutant TS N229D
Lactobacillus casei
2.1.1.45
0.15
-
2'-deoxycytidine 5'-monophosphate
mutant TS N229D
Lactobacillus casei
2.1.1.45
0.17
-
methylenetetrahydrofolate
mutant TS N229D, dCMP as substrate
Lactobacillus casei
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Lactobacillus casei
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Lactobacillus casei TS N229D
-
dihydrofolate + dTMP
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate
only mutant TS N229D
485410
Lactobacillus casei
dihydrofolate + cytidine phosphate
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate
only mutant TS N229D
485410
Lactobacillus casei TS N229D
dihydrofolate + cytidine phosphate
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei TS N229D
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
485410
Lactobacillus casei TS N229D
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
additional information
dCMP is no substrate, exception mutant TS N229D
485410
Lactobacillus casei
?
-
-
-
-
2.1.1.45
additional information
dCMP is no substrate, exception mutant TS N229D
485410
Lactobacillus casei TS N229D
?
-
-
-
-