EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.45 | medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.45 | Escherichia coli chi2913 transformed with pKPTSd containing the complete coding sequence for Lactobacillus casei TS | Lacticaseibacillus casei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Escherichia coli |
2.1.1.45 | - |
Lacticaseibacillus casei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 5-fluoro-2'-deoxyuridine 5'-monophosphate | - |
Lacticaseibacillus casei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 36000 | - |
2 * 36000 | Lacticaseibacillus casei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Escherichia coli | - |
- |
- |
2.1.1.45 | Lacticaseibacillus casei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | recombinant enzyme | Lacticaseibacillus casei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 2.5 | 3 | - |
Lacticaseibacillus casei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | dimer | - |
Escherichia coli |
2.1.1.45 | dimer | 2 * 36000 | Lacticaseibacillus casei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 16 | 35 | - |
Lacticaseibacillus casei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | (+)-methylenetetrahydrofolate | - |
Escherichia coli | |
2.1.1.45 | (+)-methylenetetrahydrofolate | - |
Lacticaseibacillus casei |