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Literature summary extracted from

  • Livi, L.L.; Edman, U.; Schneider, G.P.; Greene, P.J.; Santi, D.V.
    Cloning, expression and characterization of thymidylate synthase from Cryptococcus neoformans (1994), Gene, 150, 221-226.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine
-
 Homo sapiens
2.1.1.45 medicine
-
 Tequatrovirus T4
2.1.1.45 medicine
-
 Pneumocystis carinii
2.1.1.45 medicine drug development against cryptococcosis, a pulmonary subclinical infection, in immunocompromised individuals having diseases such as leukemia, lymphoma or AIDS the infection seminates to the central nervous system, the fourth most common opportunistic infection complicating AIDS Cryptococcus neoformans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Pneumocystis carinii
2.1.1.45 cDNA of TS gene subcloned into T7 polymerase vector pET15-b, production of active TS by complementation of Escherichia coli Cryptococcus neoformans

General Stability

EC Number General Stability Organism
2.1.1.45 stable upon quick-freezing in liquid nitrogen in buffer containing 150 mM KCl Cryptococcus neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-hydroseleno-2'-deoxyuridylic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0026
-
 2'-deoxyuridylate
-
 Cryptococcus neoformans
2.1.1.45 0.0382
-
 5,10-methylenetetrahydrofolate
-
 Cryptococcus neoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 35000
-
 SDS-PAGE Cryptococcus neoformans
2.1.1.45 35840
-
 cDNA sequence Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tequatrovirus T4
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Candida albicans
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Pneumocystis carinii biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Cryptococcus neoformans biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Cryptococcus neoformans B-3501A biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Candida albicans
-
-
-
2.1.1.45 Cryptococcus neoformans P0CS13 encapsulated yeast-like fungus, GenBank accession No.
-
2.1.1.45 Cryptococcus neoformans B-3501A P0CS13 encapsulated yeast-like fungus, GenBank accession No.
-
2.1.1.45 Homo sapiens
-
 human
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
-
-
2.1.1.45 Pneumocystis carinii
-
-
-
2.1.1.45 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 recombinant enzyme, expressed in E. coli Cryptococcus neoformans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 3
-
-
 Cryptococcus neoformans

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, 40% glycerol, stable over 2 months, 50% loss of activity by 24 h storage without glycerol Cryptococcus neoformans
2.1.1.45 -80°C, 40% glycerol, stable over 2 months Cryptococcus neoformans
2.1.1.45 -80°C, stable upon storage in Tris-HCl pH 7.4 and thiol Cryptococcus neoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Candida albicans dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Pneumocystis carinii dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Cryptococcus neoformans dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Pneumocystis carinii dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Cryptococcus neoformans dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Cryptococcus neoformans B-3501A dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Cryptococcus neoformans B-3501A dihydrofolate + dTMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7
-
-
 Mus musculus
2.1.1.45 7
-
-
 Cryptococcus neoformans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.45 6.4 8.6 50% activity at pH 6.4 and 8.6 Cryptococcus neoformans