Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Galova, M.; Koptidesova, D.; Rusznakova, D.; Racay, P.; Kollarova, M.
    Characteristics of NADPH-dependent thymidylate synthetase purified from Streptomyces aureofaciens (1992), Arch. Biochem. Biophys., 296, 81-87.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.45 beta-mercaptoethanol
-
 Mus musculus
2.1.1.45 beta-mercaptoethanol essential for maximum activity Kitasatospora aureofaciens
2.1.1.45 dithioerythritol
-
 Kitasatospora aureofaciens
2.1.1.45 dithiothreitol
-
 Mus musculus
2.1.1.45 dithiothreitol
-
 Escherichia coli
2.1.1.45 dithiothreitol
-
 Kitasatospora aureofaciens

General Stability

EC Number General Stability Organism
2.1.1.45 glycerol and Triton X-100 stabilize the enzyme Kitasatospora aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Kitasatospora aureofaciens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0033
-
 2'-deoxyuridylate
-
 Crithidia fasciculata
2.1.1.45 0.00378
-
 2'-deoxyuridylate
-
 Kitasatospora aureofaciens
2.1.1.45 0.0211
-
 N5,N10-methylenetetrahydrofolate
-
 Kitasatospora aureofaciens
2.1.1.45 0.0253
-
 methylenetetrahydrofolate
-
 Mus musculus
2.1.1.45 0.0389
-
 NADPH
-
 Kitasatospora aureofaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Na+ stimulates activity Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 28000
-
 2 * 28000, SDS-PAGE Kitasatospora aureofaciens
2.1.1.45 77000
-
 gel filtration Kitasatospora aureofaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Crithidia fasciculata
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Kitasatospora aureofaciens
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Crithidia fasciculata
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Kitasatospora aureofaciens
-
 industrial strain BMK
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-
2.1.1.45 Saccharomyces cerevisiae
-
 bakers' yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.00652
-
-
 Kitasatospora aureofaciens

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, TS activity of crude enzyme remains unchanged for 1 month of storage, purified enzyme loses 50% of its activity within 3-4 weeks Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Kitasatospora aureofaciens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Kitasatospora aureofaciens dihydrofolate + dTMP
-
 r
2.1.1.45 additional information
-
 Escherichia coli ?
-
 ?
2.1.1.45 additional information monofunctional enzyme Kitasatospora aureofaciens ?
-
 ?
2.1.1.45 [6R,S]-5,10-methylenetetrahydropteroylglutamate + 2'-deoxyuridylate
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer
-
 Saccharomyces cerevisiae
2.1.1.45 dimer
-
 Bos taurus
2.1.1.45 dimer 2 * 28000, SDS-PAGE Kitasatospora aureofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.45 30
-
-
 Crithidia fasciculata
2.1.1.45 30
-
-
 Kitasatospora aureofaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.45 15 60
-
 Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7
-
-
 Mus musculus
2.1.1.45 7
-
-
 Escherichia coli
2.1.1.45 7
-
-
 Saccharomyces cerevisiae
2.1.1.45 7
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 NADPH absolute requirement Kitasatospora aureofaciens