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Literature summary extracted from

  • Zhang, H.; Cisneros, R.J.; Deng, W.; Zapf, J.W.; Johnson, L.F.; Dunlap, R.B.
    Purification and characterization of recombinant mouse thymidylate synthase (1991), Biochim. Biophys. Acta, 1077, 35-46.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine
-
 Mus musculus
2.1.1.45 medicine
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Homo sapiens
2.1.1.45 expression vector pETSM, cloned and expressed in Escherichia coli BL21(DE3)pLysS Mus musculus

General Stability

EC Number General Stability Organism
2.1.1.45 glycerol and Triton X-100 stabilize the enzyme Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens
-
 human
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 L1210 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 recombinant enzyme Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 1.3
-
 affinity chromatography Mus musculus
2.1.1.45 1.33
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, activity remains unchanged after 1 month Mus musculus
2.1.1.45 -20°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for several weeks Mus musculus
2.1.1.45 4°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for at least 2 weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 4 9
-
 Mus musculus