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Literature summary extracted from

  • Belfort, M.; Moelleken, A.; Maley, G.F.; Maley, F.
    Purification and properties of T4 phage thymidylate synthetase produced by the cloned gene in an amplification vector (1983), J. Biol. Chem., 258, 2045-2051.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Tequatrovirus T4
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Escherichia phage T2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 T4 thymidilate synthase gene in amplification plasmid pKC30 Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 methotrexate
-
 Escherichia phage T2
2.1.1.45 methotrexate
-
 Tequatrovirus T4
2.1.1.45 Quinazoline
-
 Escherichia phage T2
2.1.1.45 Quinazoline
-
 Lacticaseibacillus casei
2.1.1.45 Quinazoline
-
 Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 32000
-
 2 * 32000, SDS-PAGE Tequatrovirus T4
2.1.1.45 32000
-
 2 * 32000, SDS-PAGE Escherichia phage T2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bacillus subtilis
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tequatrovirus T4
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia phage T2
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bacillus subtilis
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Escherichia phage T2
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Rattus norvegicus
-
 H35 hepatoma cells
-
2.1.1.45 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 purified from phage infected E. coli Tequatrovirus T4
2.1.1.45 purified from phage infected E. coli Escherichia phage T2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia phage T2 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia phage T2 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Bacillus subtilis
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer
-
 Rattus norvegicus
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 32000, SDS-PAGE Tequatrovirus T4
2.1.1.45 dimer 2 * 32000, SDS-PAGE Escherichia phage T2