EC Number | General Stability | Organism |
---|---|---|
2.1.1.45 | enzyme can be kept active for about 2-3 weeks in buffer F in presence of 0.001 mM methotrexate, dialysis against dilute buffer leads to rapid irreversible inactivation as does freeze-drying or simple freezing | Bos taurus |
2.1.1.45 | freezing and freeze-drying or dialysis causes rapid inactivation | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
2.1.1.45 | tetrahydromethotrexate | - |
Bos taurus | |
2.1.1.45 | tetrahydromethotrexate | - |
Escherichia coli | |
2.1.1.45 | tetrahydromethotrexate | - |
Lacticaseibacillus casei | |
2.1.1.45 | tetrahydromethotrexate | - |
Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 35000 | - |
2 * 35000, SDS-PAGE | Mus musculus |
2.1.1.45 | 35000 | - |
2 * 35000, SDS-PAGE | Bos taurus |
2.1.1.45 | 70000 | - |
- |
Mus musculus |
2.1.1.45 | 70000 | - |
gel filtration | Escherichia coli |
2.1.1.45 | 70000 | - |
gel filtration | Bos taurus |
2.1.1.45 | 70000 | - |
gel filtration | Lacticaseibacillus casei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | - |
dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Bos taurus | - |
calf | - |
2.1.1.45 | Escherichia coli | - |
- |
- |
2.1.1.45 | Escherichia coli B / ATCC 11303 | - |
- |
- |
2.1.1.45 | Lacticaseibacillus casei | - |
- |
- |
2.1.1.45 | Mus musculus | - |
Gardner lymphosarcoma | - |
2.1.1.45 | Mus musculus | - |
mouse, sarcoma 180 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Lacticaseibacillus casei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.45 | thymus | - |
Mus musculus | - |
2.1.1.45 | thymus | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 0.002135 | - |
Gardner lymphosarcoma | Mus musculus |
2.1.1.45 | 0.003519 | - |
sarcoma 180 | Mus musculus |
2.1.1.45 | 0.1024 | - |
- |
Bos taurus |
2.1.1.45 | 0.6778 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.45 | -20°C, occasionally loses activity | Mus musculus |
2.1.1.45 | -50°C-to -20°C, activity of purified enzyme remains unchanged for 2-3 months | Bos taurus |
2.1.1.45 | 0-10°C enzyme is stable in buffer D for several weeks | Escherichia coli |
2.1.1.45 | 0-7°C, concentrated enzyme keeps its activity for 14 days | Bos taurus |
2.1.1.45 | 0°C, enzyme rapidly loses its activity, more than 90% in 2 h | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | (6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate | - |
Lacticaseibacillus casei | 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | dimer | - |
Escherichia coli |
2.1.1.45 | dimer | - |
Lacticaseibacillus casei |
2.1.1.45 | dimer | 2 * 35000, SDS-PAGE | Mus musculus |
2.1.1.45 | dimer | 2 * 35000, SDS-PAGE | Bos taurus |