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Literature summary extracted from

  • Al Chalabi, K.; Gutteridge, W.E.
    Presence and properties of thymidylate synthase in trypanosomatids (1977), Biochim. Biophys. Acta, 481, 71-79.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine possible target for chemotherapeutic attack Trypanosoma brucei
2.1.1.45 medicine possible target for chemotherapeutic attack Crithidia fasciculata
2.1.1.45 medicine possible target for chemotherapeutic attack Trypanosoma cruzi
2.1.1.45 medicine possible target for chemotherapeutic attack Trypanosoma congolense
2.1.1.45 medicine possible target for chemotherapeutic attack Strigomonas oncopelti
2.1.1.45 medicine possible target for chemotherapeutic attack Trypanosoma lewisi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 2-mercaptoethanol
-
 Crithidia fasciculata
2.1.1.45 2-mercaptoethanol
-
 Strigomonas oncopelti
2.1.1.45 2-mercaptoethanol
-
 Trypanosoma brucei
2.1.1.45 2-mercaptoethanol
-
 Trypanosoma congolense
2.1.1.45 2-mercaptoethanol
-
 Trypanosoma cruzi
2.1.1.45 2-mercaptoethanol
-
 Trypanosoma lewisi
2.1.1.45 5-fluorouracil
-
 Crithidia fasciculata
2.1.1.45 5-fluorouracil
-
 Escherichia coli
2.1.1.45 5-fluorouracil
-
 Trypanosoma cruzi
2.1.1.45 aminopterin
-
 Crithidia fasciculata
2.1.1.45 aminopterin
-
 Trypanosoma cruzi
2.1.1.45 antrycide
-
 Crithidia fasciculata
2.1.1.45 antrycide
-
 Strigomonas oncopelti
2.1.1.45 antrycide
-
 Trypanosoma brucei
2.1.1.45 antrycide
-
 Trypanosoma congolense
2.1.1.45 antrycide
-
 Trypanosoma cruzi
2.1.1.45 antrycide
-
 Trypanosoma lewisi
2.1.1.45 dimidium
-
 Crithidia fasciculata
2.1.1.45 dimidium
-
 Strigomonas oncopelti
2.1.1.45 dimidium
-
 Trypanosoma brucei
2.1.1.45 dimidium
-
 Trypanosoma congolense
2.1.1.45 dimidium
-
 Trypanosoma cruzi
2.1.1.45 dimidium
-
 Trypanosoma lewisi
2.1.1.45 ethidium
-
 Crithidia fasciculata
2.1.1.45 ethidium
-
 Strigomonas oncopelti
2.1.1.45 ethidium
-
 Trypanosoma brucei
2.1.1.45 ethidium
-
 Trypanosoma congolense
2.1.1.45 ethidium
-
 Trypanosoma cruzi
2.1.1.45 ethidium
-
 Trypanosoma lewisi
2.1.1.45 Mg2+
-
 Crithidia fasciculata
2.1.1.45 Mg2+
-
 Strigomonas oncopelti
2.1.1.45 Mg2+
-
 Trypanosoma brucei
2.1.1.45 Mg2+
-
 Trypanosoma congolense
2.1.1.45 Mg2+
-
 Trypanosoma cruzi
2.1.1.45 Mg2+
-
 Trypanosoma lewisi
2.1.1.45 Pentamidine
-
 Crithidia fasciculata
2.1.1.45 Pentamidine
-
 Strigomonas oncopelti
2.1.1.45 Pentamidine
-
 Trypanosoma brucei
2.1.1.45 Pentamidine
-
 Trypanosoma congolense
2.1.1.45 Pentamidine
-
 Trypanosoma cruzi
2.1.1.45 Pentamidine
-
 Trypanosoma lewisi
2.1.1.45 prothidium
-
 Crithidia fasciculata
2.1.1.45 prothidium
-
 Strigomonas oncopelti
2.1.1.45 prothidium
-
 Trypanosoma brucei
2.1.1.45 prothidium
-
 Trypanosoma congolense
2.1.1.45 prothidium
-
 Trypanosoma cruzi
2.1.1.45 prothidium
-
 Trypanosoma lewisi
2.1.1.45 suramin
-
 Crithidia fasciculata
2.1.1.45 suramin
-
 Strigomonas oncopelti
2.1.1.45 suramin
-
 Trypanosoma brucei
2.1.1.45 suramin
-
 Trypanosoma congolense
2.1.1.45 suramin
-
 Trypanosoma cruzi
2.1.1.45 suramin
-
 Trypanosoma lewisi
2.1.1.45 trimethoprim
-
 Crithidia fasciculata
2.1.1.45 trimethoprim
-
 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 175000
-
 gel filtration Crithidia fasciculata
2.1.1.45 200000
-
 gel filtration Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma brucei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Crithidia fasciculata
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma cruzi
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma congolense
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Strigomonas oncopelti
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma lewisi
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma brucei gambiense
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Trypanosoma rhodesiense
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Crithidia fasciculata
-
 host Anopheles strain ATCC 11,745
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Rattus norvegicus
-
-
-
2.1.1.45 Strigomonas oncopelti
-
-
-
2.1.1.45 Trypanosoma brucei
-
-
-
2.1.1.45 Trypanosoma brucei gambiense
-
-
-
2.1.1.45 Trypanosoma congolense
-
 causes nagana, the equivalent disease of cattle
-
2.1.1.45 Trypanosoma cruzi
-
 sonya strain, maintained in ASH/XP mice
-
2.1.1.45 Trypanosoma cruzi
-
 causes Chagas' disease in man
-
2.1.1.45 Trypanosoma lewisi
-
 natural pathogen of rats
-
2.1.1.45 Trypanosoma rhodesiense
-
 causes human sleeping sickness
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 blood intracellular parasites, host blood Trypanosoma brucei
-
2.1.1.45 blood intracellular parasites, host blood Crithidia fasciculata
-
2.1.1.45 blood intracellular parasites, host blood Trypanosoma cruzi
-
2.1.1.45 blood intracellular parasites, host blood Trypanosoma congolense
-
2.1.1.45 blood intracellular parasites, host blood Strigomonas oncopelti
-
2.1.1.45 blood intracellular parasites, host blood Trypanosoma lewisi
-
2.1.1.45 blood obtained from infected rat blood Trypanosoma brucei
-
2.1.1.45 blood obtained from infected rat blood Trypanosoma congolense
-
2.1.1.45 blood obtained from infected rat blood Trypanosoma lewisi
-
2.1.1.45 fetus
-
 Rattus norvegicus
-
2.1.1.45 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.00012
-
-
 Trypanosoma brucei
2.1.1.45 0.00014
-
-
 Trypanosoma congolense
2.1.1.45 0.00035
-
 intracellular form Trypanosoma cruzi
2.1.1.45 0.00038
-
-
 Trypanosoma lewisi
2.1.1.45 0.00054
-
-
 Strigomonas oncopelti
2.1.1.45 0.00119
-
 blood form Trypanosoma cruzi
2.1.1.45 0.00156
-
 culture form Trypanosoma cruzi
2.1.1.45 0.00218
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma brucei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma brucei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Crithidia fasciculata dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma cruzi dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma cruzi dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma congolense dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma congolense dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Strigomonas oncopelti dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Strigomonas oncopelti dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma lewisi dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma lewisi dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma brucei gambiense dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma brucei gambiense dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma rhodesiense dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma rhodesiense dihydrofolate + dTMP
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.45 30
-
-
 Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.5 6.7
-
 Trypanosoma lewisi
2.1.1.45 6.5 6.7
-
 Trypanosoma brucei gambiense
2.1.1.45 7.8
-
-
 Crithidia fasciculata