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Literature summary extracted from

  • McCuen, R.W.; Sirotnak, F.M.
    Thymidylate synthetase from Diplococcus pneumoniae, properties and inhibition by folate analogs (1975), Biochim. Biophys. Acta, 384, 369-380.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 2,4-diamino pteridine
-
 Streptococcus pneumoniae
2.1.1.45 2,4-diamino quinazoline antifolate
-
 Streptococcus pneumoniae
2.1.1.45 2-amino-4-hydroxyquinazoline
-
 Streptococcus pneumoniae
2.1.1.45 aminopterin
-
 Streptococcus pneumoniae
2.1.1.45 deaza-aminopterin
-
 Streptococcus pneumoniae
2.1.1.45 methasquin
-
 Streptococcus pneumoniae
2.1.1.45 methopterin
-
 Escherichia coli
2.1.1.45 methopterin
-
 Streptococcus pneumoniae
2.1.1.45 quinaspar
-
 Streptococcus pneumoniae
2.1.1.45 SK 28,757
-
 Streptococcus pneumoniae
2.1.1.45 SK 28,758
-
 Streptococcus pneumoniae
2.1.1.45 SK 28,815
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0308
-
 2'-deoxyuridylate
-
 Streptococcus pneumoniae
2.1.1.45 0.266
-
 5,10-methylenetetrahydrofolate
-
 Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Mg2+
-
 Escherichia coli
2.1.1.45 Mg2+
-
 Lacticaseibacillus casei
2.1.1.45 Mg2+ requirement depending upon the buffer Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 34000 38000 gel filtration, sucrose density-gradient centrifugation Streptococcus pneumoniae
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Lacticaseibacillus casei
2.1.1.45 58000
-
-
 Gallus gallus
2.1.1.45 70000
-
-
 Escherichia coli
2.1.1.45 70000
-
-
 Streptococcus pneumoniae
2.1.1.45 70000
-
-
 Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Streptococcus pneumoniae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecium
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Enterococcus faecium
-
 var. durans
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Gallus gallus
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Streptococcus pneumoniae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.002
-
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Streptococcus pneumoniae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Streptococcus pneumoniae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterococcus faecium dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterococcus faecium dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 r
2.1.1.45 additional information uridylate, cytidylate and deoxycytidylate are not substrates Streptococcus pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer 2 * 35000, SDS-PAGE Lacticaseibacillus casei
2.1.1.45 dimer 2 * 34000-38000 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7 7.7
-
 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 5 8
-
 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.0013
-
 SK 28,757
-
 Streptococcus pneumoniae
2.1.1.45 0.0013
-
 2,4-diamino quinazoline
-
 Streptococcus pneumoniae
2.1.1.45 0.0015
-
 SK 28,758
-
 Streptococcus pneumoniae
2.1.1.45 0.0029
-
 methasquin
-
 Streptococcus pneumoniae
2.1.1.45 0.0029
-
 SK 28,815
-
 Streptococcus pneumoniae
2.1.1.45 0.003
-
 2,4-diamino quinazoline antifolate
-
 Streptococcus pneumoniae
2.1.1.45 0.0044
-
 quinaspar
-
 Streptococcus pneumoniae
2.1.1.45 0.0075
-
 deaza-aminopterin
-
 Streptococcus pneumoniae
2.1.1.45 0.0284
-
 methopterin
-
 Streptococcus pneumoniae
2.1.1.45 0.03
-
 2,4-diamino pteridine
-
 Streptococcus pneumoniae
2.1.1.45 0.0347
-
 aminopterin
-
 Streptococcus pneumoniae