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Literature summary extracted from

  • Capco, G.R.; Krupp, J.R.; Mathews, C.K.
    Bacteriophage-coded thymidylate synthetase: characteristics of the T4 and T5 enzymes (1973), Arch. Biochem. Biophys., 158, 726-735.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.45 quite unstable Tequatrovirus T4
2.1.1.45 quite unstable Tequintavirus T5

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Tequatrovirus T4
2.1.1.45 trifluoromethyldeoxyuridine 5'-monophosphate
-
 Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 29000
-
 2 * 29000, SDS-PAGE Tequatrovirus T4
2.1.1.45 55000
-
 gel filtration Tequintavirus T5
2.1.1.45 58000
-
 sucrose density gradient analysis Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tequatrovirus T4
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tequintavirus T5
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Tequatrovirus T4
-
-
-
2.1.1.45 Tequintavirus T5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 purified from phage infected E. coli Tequatrovirus T4
2.1.1.45 purified from phage infected E. coli Tequintavirus T5

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 14.2
-
-
 Tequintavirus T5
2.1.1.45 14.45
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequatrovirus T4 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequintavirus T5 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tequintavirus T5 dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 29000, SDS-PAGE Tequatrovirus T4