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Literature summary extracted from

  • Santi, D.V.; Sakai, T.T.
    Irreversible inhibition of thymidylate synthetase by 5-formyl-2-deoxyuridylic acid (1972), Biochem. Biophys. Res. Commun., 46, 1320-1325.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.45 enzyme is significantly stabilized by dUMP Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-Formyl-2'-deoxyuridylic acid
-
Escherichia coli
2.1.1.45 5-Formyl-2'-deoxyuridylic acid
-
Lacticaseibacillus casei
2.1.1.45 methopterin
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303
-
dihydrofolate + dTMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
Ehrlich ascites carcinoma cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.45 25
-
rather unstable Escherichia coli
2.1.1.45 25
-
considerably stable, no loss of activity as long as 50 min Lacticaseibacillus casei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000012
-
5-Formyl-2'-deoxyuridylic acid
-
Lacticaseibacillus casei
2.1.1.45 0.000013
-
5-Formyl-2'-deoxyuridylic acid
-
Escherichia coli