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Literature summary extracted from

  • Fridland, A.; Langenbach, R.J.; Heidelberger, C.
    Purification of thymidylate synthetase from Ehrlich ascites carcinoma cells (1971), J. Biol. Chem., 246, 7110-7114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.45 beta-mercaptoethanol
-
Mus musculus
2.1.1.45 dithiothreitol
-
Escherichia coli
2.1.1.45 dithiothreitol
-
Homo sapiens
2.1.1.45 dithiothreitol thiols enhances enzyme activity Mus musculus

General Stability

EC Number General Stability Organism
2.1.1.45 enzyme is not inactivated by freezing and thawing Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
Enterococcus faecalis
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
Mus musculus
2.1.1.45 p-chloromercuribenzoate
-
Lacticaseibacillus casei
2.1.1.45 trifluoromethyldeoxyuridine 5'-monophosphate
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0063
-
2'-deoxyuridylate
-
Mus musculus
2.1.1.45 0.043
-
5,10-methylenetetrahydrofolate
-
Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 58000
-
-
Gallus gallus
2.1.1.45 67000
-
-
Lacticaseibacillus casei
2.1.1.45 67000
-
Ehrlich ascites carcinoma cells, gel filtration Mus musculus
2.1.1.45 70000
-
-
Mus musculus
2.1.1.45 70000
-
-
Escherichia coli
2.1.1.45 70000
-
-
Bos taurus
2.1.1.45 70000
-
-
Lacticaseibacillus casei
2.1.1.45 71000
-
-
Lacticaseibacillus casei
2.1.1.45 71000
-
Ehrlich ascites carcinoma cells, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bos taurus
-
calf
-
2.1.1.45 Enterococcus faecalis
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Gallus gallus
-
chicken
-
2.1.1.45 Homo sapiens
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
albino Swiss mice
-
2.1.1.45 Mus musculus
-
Ehrlich ascites carcinoma cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 embryo
-
Gallus gallus
-
2.1.1.45 thymus
-
Mus musculus
-
2.1.1.45 thymus
-
Homo sapiens
-
2.1.1.45 thymus
-
Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, sulfate-precipitated enzyme in 0.1 M potassium phosphate, 0.01 M dithiothreitol, pH 7.1can be kept at least 4 months with only 25% loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Enterococcus faecalis dihydrofolate + dTMP
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 monomer 1 * 71000, Ehrlich ascites carcinoma cells, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.7
-
-
Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 6 8
-
Mus musculus