EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | beta-mercaptoethanol | - |
Mus musculus | |
2.1.1.45 | dithiothreitol | - |
Escherichia coli | |
2.1.1.45 | dithiothreitol | - |
Homo sapiens | |
2.1.1.45 | dithiothreitol | thiols enhances enzyme activity | Mus musculus |
EC Number | General Stability | Organism |
---|---|---|
2.1.1.45 | enzyme is not inactivated by freezing and thawing | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Enterococcus faecalis | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
2.1.1.45 | p-chloromercuribenzoate | - |
Lacticaseibacillus casei | |
2.1.1.45 | trifluoromethyldeoxyuridine 5'-monophosphate | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.45 | 0.0063 | - |
2'-deoxyuridylate | - |
Mus musculus | |
2.1.1.45 | 0.043 | - |
5,10-methylenetetrahydrofolate | - |
Enterococcus faecalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 58000 | - |
- |
Gallus gallus |
2.1.1.45 | 67000 | - |
- |
Lacticaseibacillus casei |
2.1.1.45 | 67000 | - |
Ehrlich ascites carcinoma cells, gel filtration | Mus musculus |
2.1.1.45 | 70000 | - |
- |
Mus musculus |
2.1.1.45 | 70000 | - |
- |
Escherichia coli |
2.1.1.45 | 70000 | - |
- |
Bos taurus |
2.1.1.45 | 70000 | - |
- |
Lacticaseibacillus casei |
2.1.1.45 | 71000 | - |
- |
Lacticaseibacillus casei |
2.1.1.45 | 71000 | - |
Ehrlich ascites carcinoma cells, SDS-PAGE | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Bos taurus | - |
calf | - |
2.1.1.45 | Enterococcus faecalis | - |
- |
- |
2.1.1.45 | Escherichia coli | - |
- |
- |
2.1.1.45 | Gallus gallus | - |
chicken | - |
2.1.1.45 | Homo sapiens | - |
- |
- |
2.1.1.45 | Lacticaseibacillus casei | - |
- |
- |
2.1.1.45 | Mus musculus | - |
albino Swiss mice | - |
2.1.1.45 | Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | Ehrlich ascites carcinoma cells | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.45 | embryo | - |
Gallus gallus | - |
2.1.1.45 | thymus | - |
Mus musculus | - |
2.1.1.45 | thymus | - |
Homo sapiens | - |
2.1.1.45 | thymus | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.45 | -20°C, sulfate-precipitated enzyme in 0.1 M potassium phosphate, 0.01 M dithiothreitol, pH 7.1can be kept at least 4 months with only 25% loss of activity | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Enterococcus faecalis | dihydrofolate + dTMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | monomer | 1 * 71000, Ehrlich ascites carcinoma cells, SDS-PAGE | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 6.7 | - |
- |
Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 6 | 8 | - |
Mus musculus |