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Literature summary extracted from

  • Blakeley, R.L.
    The biosythesis of thymidylic acid. IV. Further studies on thymidylate synthetase (1963), J. Biol. Chem., 238, 2113-2118.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.45 2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Escherichia coli
2.1.1.45 2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Homo sapiens
2.1.1.45 2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Enterococcus faecalis
2.1.1.45 KCN
-
 Escherichia coli
2.1.1.45 KCN
-
 Homo sapiens
2.1.1.45 KCN
-
 Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridine 5'-(p-aminophenyl phosphate)
-
 Enterococcus faecalis
2.1.1.45 5-fluoro-2'-deoxyuridylic acid very strong inhibition, pseudo-noncompetitive Enterococcus faecalis
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 dihydrofolate
-
 Enterococcus faecalis
2.1.1.45 dihydrofolate
-
 Escherichia coli
2.1.1.45 dTMP
-
 Enterococcus faecalis
2.1.1.45 methotrexate
-
 Homo sapiens
2.1.1.45 thymidine phosphate product inhibition Enterococcus faecalis
2.1.1.45 thymidine phosphate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0012
-
 2'-deoxyuridylate
-
 Escherichia coli
2.1.1.45 0.0057
-
 2'-deoxyuridylate
-
 Enterococcus faecalis
2.1.1.45 0.016
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
2.1.1.45 0.018
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
2.1.1.45 0.035
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
2.1.1.45 0.037
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
2.1.1.45 0.039
-
 5,10-methylenetetrahydrofolate
-
 Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Enterococcus faecalis
-
 synonym Enterococcus faecalis, strain R
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 partially Homo sapiens
2.1.1.45 partially Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Escherichia coli 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Homo sapiens 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Enterococcus faecalis 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Enterococcus faecalis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Enterococcus faecalis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Enterococcus faecalis dihydrofolate + dTMP
-
 ?
2.1.1.45 additional information
-
 Escherichia coli ?
-
 ?
2.1.1.45 additional information
-
 Homo sapiens ?
-
 ?
2.1.1.45 additional information UMP, dCMP and deoxyuridine are no substrates Enterococcus faecalis ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000004
-
 5-fluoro-2'-deoxyuridylic acid
-
 Enterococcus faecalis
2.1.1.45 0.023
-
 methotrexate uncompetitive with methylene tetrahydrofolate Homo sapiens
2.1.1.45 0.024
-
 dTMP
-
 Enterococcus faecalis