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Literature summary extracted from

  • Wahba, A.J.; Friedkin, M.
    The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli (1962), J. Biol. Chem., 237, 3794-3801.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 Chloroacetamide
-
Escherichia coli
2.1.1.45 iodoacetate
-
Escherichia coli
2.1.1.45 N-ethylmaleimide
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.004
-
2'-deoxyuridylate
-
Escherichia coli
2.1.1.45 0.0045
-
L-tetrahydrofolate
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303
-
dihydrofolate + dTMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Enterococcus faecalis
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.016
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 (-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate
-
Escherichia coli 7,8-dihydropteroylglutamate + thymidine phosphate
-
?
2.1.1.45 (-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate
-
Escherichia coli B / ATCC 11303 7,8-dihydropteroylglutamate + thymidine phosphate
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Enterococcus faecalis dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7 8 activity is higher in Tris buffer than in phosphate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 5.5 7.8 phosphate buffer Escherichia coli
2.1.1.45 6.2 9.5 Tris buffer Escherichia coli