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Literature summary extracted from

  • Cantoni, G.L.; Vignos, P.J.
    Enzymatic mechanism of creatine synthesis (1954), J. Biol. Chem., 209, 647-659.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.2 CN-
-
 Cavia porcellus
2.1.1.2 CN-
-
 Sus scrofa
2.1.1.2 CN-
-
 Bos taurus
2.1.1.2 CN-
-
 Oryctolagus cuniculus
2.1.1.2 cysteine
-
 Cavia porcellus
2.1.1.2 cysteine
-
 Sus scrofa
2.1.1.2 cysteine
-
 Bos taurus
2.1.1.2 cysteine
-
 Oryctolagus cuniculus
2.1.1.2 glutathione required for maximum activity, can be replaced by other reducing agents Cavia porcellus
2.1.1.2 glutathione required for maximum activity, can be replaced by other reducing agents Sus scrofa
2.1.1.2 glutathione required for maximum activity, can be replaced by other reducing agents Bos taurus
2.1.1.2 glutathione required for maximum activity, can be replaced by other reducing agents Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.2 p-chloromercuribenzoate complete inhibition at 1 mM, reversible by addition of glutathione Bos taurus
2.1.1.2 p-chloromercuribenzoate complete inhibition at 1 mM, reversible by addition of glutathione Cavia porcellus
2.1.1.2 p-chloromercuribenzoate complete inhibition at 1 mM, reversible by addition of glutathione Oryctolagus cuniculus
2.1.1.2 p-chloromercuribenzoate complete inhibition at 1 mM, reversible by addition of glutathione Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.2 Mg2+
-
 Cavia porcellus
2.1.1.2 Mg2+
-
 Sus scrofa
2.1.1.2 Mg2+
-
 Bos taurus
2.1.1.2 Mg2+
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Cavia porcellus biosynthesis of creatine S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Sus scrofa biosynthesis of creatine S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Bos taurus biosynthesis of creatine S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Oryctolagus cuniculus biosynthesis of creatine S-adenosyl-L-homocysteine + creatine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.2 Bos taurus
-
-
-
2.1.1.2 Cavia porcellus
-
-
-
2.1.1.2 Oryctolagus cuniculus
-
-
-
2.1.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.2 liver
-
 Cavia porcellus
-
2.1.1.2 liver
-
 Sus scrofa
-
2.1.1.2 liver
-
 Bos taurus
-
2.1.1.2 liver
-
 Oryctolagus cuniculus
-
2.1.1.2 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.2 0.022
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate S-adenosyl-L-methionine is the only possible methyl donor Cavia porcellus S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate S-adenosyl-L-methionine is the only possible methyl donor Sus scrofa S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate S-adenosyl-L-methionine is the only possible methyl donor Bos taurus S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate S-adenosyl-L-methionine is the only possible methyl donor Oryctolagus cuniculus S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate biosynthesis of creatine Cavia porcellus S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate biosynthesis of creatine Sus scrofa S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate biosynthesis of creatine Bos taurus S-adenosyl-L-homocysteine + creatine
-
 ?
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate biosynthesis of creatine Oryctolagus cuniculus S-adenosyl-L-homocysteine + creatine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.2 7.5
-
-
 Cavia porcellus
2.1.1.2 7.5
-
-
 Sus scrofa
2.1.1.2 7.5
-
-
 Bos taurus
2.1.1.2 7.5
-
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.2 ATP
-
 Cavia porcellus
2.1.1.2 ATP
-
 Sus scrofa
2.1.1.2 ATP
-
 Bos taurus
2.1.1.2 ATP
-
 Oryctolagus cuniculus