BRENDA - Enzyme Database

Phosphatidylethanolamine N-methyltransferase activity in isolated rod outer segments from bovine retina

Roque, M.E.; Giusto, N.M.; Exp. Eye Res. 60, 631-643 (1995)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.037
-
S-adenosylmethionine
at pH 8.5
Bos taurus
2.1.1.17
0.038
-
S-adenosylmethionine
at pH 10
Bos taurus
2.1.1.71
0.037
-
S-adenosyl-L-methionine
pH 8.5
Bos taurus
2.1.1.71
0.038
-
S-adenosyl-L-methionine
pH 10.0
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.17
membrane
intrinsic membrane protein
Bos taurus
16020
-
2.1.1.71
cytosol
-
Bos taurus
5829
-
2.1.1.71
membrane
synaptosomal plasma membrane
Rattus norvegicus
16020
-
2.1.1.71
microsome
-
Bos taurus
-
-
2.1.1.71
microsome
-
Rattus norvegicus
-
-
2.1.1.71
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.17
Mg2+
required, maximal activity at 10 mM
Bos taurus
2.1.1.71
Mg2+
requires magnesium ions for full activity
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.17
Bos taurus
-
-
-
2.1.1.71
Bos taurus
-
bovine
-
2.1.1.71
Rattus norvegicus
-
rat
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.71
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
retina
rod outer segment
Bos taurus
-
2.1.1.71
eye
-
Bos taurus
-
2.1.1.71
liver
-
Rattus norvegicus
-
2.1.1.71
retina
rod outer segments
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
8
-
at low concentrations of S-adenosylmethionine, 0.01 mM
Bos taurus
2.1.1.17
10
-
at high concentrations of S-adenosylmethionine, 0.2 mM
Bos taurus
2.1.1.71
8.5
-
low substrate concentrations about 0.01 M S-adenosyl-L-methionine
Bos taurus
2.1.1.71
10
-
high substrate concentrations about 0.2 M S-adenosyl-L-methionine
Bos taurus
2.1.1.71
10
-
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.71
7
11
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.037
-
S-adenosylmethionine
at pH 8.5
Bos taurus
2.1.1.17
0.038
-
S-adenosylmethionine
at pH 10
Bos taurus
2.1.1.71
0.037
-
S-adenosyl-L-methionine
pH 8.5
Bos taurus
2.1.1.71
0.038
-
S-adenosyl-L-methionine
pH 10.0
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.17
membrane
intrinsic membrane protein
Bos taurus
16020
-
2.1.1.71
cytosol
-
Bos taurus
5829
-
2.1.1.71
membrane
synaptosomal plasma membrane
Rattus norvegicus
16020
-
2.1.1.71
microsome
-
Bos taurus
-
-
2.1.1.71
microsome
-
Rattus norvegicus
-
-
2.1.1.71
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.17
Mg2+
required, maximal activity at 10 mM
Bos taurus
2.1.1.71
Mg2+
requires magnesium ions for full activity
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.71
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
retina
rod outer segment
Bos taurus
-
2.1.1.71
eye
-
Bos taurus
-
2.1.1.71
liver
-
Rattus norvegicus
-
2.1.1.71
retina
rod outer segments
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
2.1.1.71
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
8
-
at low concentrations of S-adenosylmethionine, 0.01 mM
Bos taurus
2.1.1.17
10
-
at high concentrations of S-adenosylmethionine, 0.2 mM
Bos taurus
2.1.1.71
8.5
-
low substrate concentrations about 0.01 M S-adenosyl-L-methionine
Bos taurus
2.1.1.71
10
-
high substrate concentrations about 0.2 M S-adenosyl-L-methionine
Bos taurus
2.1.1.71
10
-
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.71
7
11
-
Bos taurus