BRENDA - Enzyme Database

Purification and characterization of phosphatidylethanolamine N-methyltransferase from Zymomonas mobilis

Tahara, Y.; Ogawa, Y.; Sakakibara, T.; Yamada, Y.; Agric. Biol. Chem. 51, 1425-1430 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.17
L-Cys
enhances activity, optimal concentration 10 mM
Zymomonas mobilis
2.1.1.17
Triton X-100
enhances activity, optimal concentration 0.04%
Zymomonas mobilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.17
S-adenosylhomocysteine
competitive with respect to S-adenosylmethionine
Zymomonas mobilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.0019
-
S-adenosylmethionine
reaction with phosphatidyl-N-monomethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0036
-
S-adenosylmethionine
reaction with phosphatidylethanolamine
Zymomonas mobilis
2.1.1.17
0.0039
-
S-adenosylmethionine
phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0175
-
phosphatidyl-N-monomethylethanolamine
-
Zymomonas mobilis
2.1.1.17
0.0336
-
phosphatidylethanolamine
-
Zymomonas mobilis
2.1.1.17
0.0405
-
phosphatidyl-N-monomethylethanolamine
-
Zymomonas mobilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.17
42000
-
3 * 42000, SDS-PAGE
Zymomonas mobilis
2.1.1.17
120000
-
gel filtration
Zymomonas mobilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
-
S-adenosyl-L-homocysteine + phosphatidylcholine
-
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
Zymomonas mobilis
-
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
Zymomonas mobilis
the first-step methylation from phosphatidylethanolamine to phosphatidylcholine is rate-limiting
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.17
Zymomonas mobilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.17
-
Zymomonas mobilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
additional information
-
-
Zymomonas mobilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidylcholine
-
485175
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
485175
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
the first-step methylation from phosphatidylethanolamine to phosphatidylcholine is rate-limiting
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.17
trimer
3 * 42000, SDS-PAGE
Zymomonas mobilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
8.5
-
-
Zymomonas mobilis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.17
0.0048
-
S-adenosyl-L-homocysteine
towards phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0087
-
S-adenosyl-L-homocysteine
towards phosphatidyl-N-monomethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0118
-
S-adenosyl-L-homocysteine
towards phosphatidylethanolamine
Zymomonas mobilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.17
L-Cys
enhances activity, optimal concentration 10 mM
Zymomonas mobilis
2.1.1.17
Triton X-100
enhances activity, optimal concentration 0.04%
Zymomonas mobilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.17
S-adenosylhomocysteine
competitive with respect to S-adenosylmethionine
Zymomonas mobilis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.17
0.0048
-
S-adenosyl-L-homocysteine
towards phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0087
-
S-adenosyl-L-homocysteine
towards phosphatidyl-N-monomethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0118
-
S-adenosyl-L-homocysteine
towards phosphatidylethanolamine
Zymomonas mobilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.0019
-
S-adenosylmethionine
reaction with phosphatidyl-N-monomethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0036
-
S-adenosylmethionine
reaction with phosphatidylethanolamine
Zymomonas mobilis
2.1.1.17
0.0039
-
S-adenosylmethionine
phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
2.1.1.17
0.0175
-
phosphatidyl-N-monomethylethanolamine
-
Zymomonas mobilis
2.1.1.17
0.0336
-
phosphatidylethanolamine
-
Zymomonas mobilis
2.1.1.17
0.0405
-
phosphatidyl-N-monomethylethanolamine
-
Zymomonas mobilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.17
42000
-
3 * 42000, SDS-PAGE
Zymomonas mobilis
2.1.1.17
120000
-
gel filtration
Zymomonas mobilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
Zymomonas mobilis
-
S-adenosyl-L-homocysteine + phosphatidylcholine
-
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
Zymomonas mobilis
-
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
Zymomonas mobilis
the first-step methylation from phosphatidylethanolamine to phosphatidylcholine is rate-limiting
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.17
-
Zymomonas mobilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
additional information
-
-
Zymomonas mobilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidylcholine
-
485175
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
485175
Zymomonas mobilis
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
the first-step methylation from phosphatidylethanolamine to phosphatidylcholine is rate-limiting
485175
Zymomonas mobilis
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.17
trimer
3 * 42000, SDS-PAGE
Zymomonas mobilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
8.5
-
-
Zymomonas mobilis