BRENDA - Enzyme Database

Phosphatidylethanolamine N-methyltransferase from rat liver

Ridgway, N.D.; Vance, D.E.; Methods Enzymol. 209, 366-374 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.17
additional information
phosphatidylethanolamine, monomethyl-phosphatidylethanolamine and dimethyl-phosphatidylethanolamine compete for a common active site
Rattus norvegicus
2.1.1.17
S-adenosylhomocysteine
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.016
-
S-adenosylmethionine
reaction with phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
2.1.1.17
0.022
-
S-adenosylmethionine
reaction with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
0.08
-
phosphatidyl-N-monomethylethanolamine
-
Rattus norvegicus
2.1.1.17
0.45
-
phosphatidyl-N,N-dimethylethanolamine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.17
endoplasmic reticulum
-
Rattus norvegicus
5783
-
2.1.1.17
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.17
18300
-
1 * 18300, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.17
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
0.63
-
activity with phosphatidylethanolamine
Rattus norvegicus
2.1.1.17
3.75
-
activity with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
8.59
-
activity with phosphatidyl-N,N-dimethyl-ethanolamine
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
ordered bi-bi mechanism
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
ordered bi-bi mechanism
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
transfer of three methyl groups to the amino head group of phosphatidylethanolamine
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.17
monomer
1 * 18300, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
additional information
-
activity increases in nearly a linear fashion between pH 6.0 and 9.5
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.17
0.0049
-
S-adenosyl-L-homocysteine
reaction with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
0.0067
-
S-adenosyl-L-homocysteine
reaction with phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
2.1.1.17
0.0739
-
S-adenosylhomocysteine
reaction with phosphatidyl-N-dimethylethanolamine
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.17
additional information
phosphatidylethanolamine, monomethyl-phosphatidylethanolamine and dimethyl-phosphatidylethanolamine compete for a common active site
Rattus norvegicus
2.1.1.17
S-adenosylhomocysteine
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.17
0.0049
-
S-adenosyl-L-homocysteine
reaction with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
0.0067
-
S-adenosyl-L-homocysteine
reaction with phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
2.1.1.17
0.0739
-
S-adenosylhomocysteine
reaction with phosphatidyl-N-dimethylethanolamine
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.17
0.016
-
S-adenosylmethionine
reaction with phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
2.1.1.17
0.022
-
S-adenosylmethionine
reaction with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
0.08
-
phosphatidyl-N-monomethylethanolamine
-
Rattus norvegicus
2.1.1.17
0.45
-
phosphatidyl-N,N-dimethylethanolamine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.17
endoplasmic reticulum
-
Rattus norvegicus
5783
-
2.1.1.17
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.17
18300
-
1 * 18300, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.17
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
0.63
-
activity with phosphatidylethanolamine
Rattus norvegicus
2.1.1.17
3.75
-
activity with phosphatidyl-N-monomethylethanolamine
Rattus norvegicus
2.1.1.17
8.59
-
activity with phosphatidyl-N,N-dimethyl-ethanolamine
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
ordered bi-bi mechanism
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
ordered bi-bi mechanism
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
2.1.1.17
S-adenosyl-L-methionine + phosphatidylethanolamine
transfer of three methyl groups to the amino head group of phosphatidylethanolamine
485164
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.17
monomer
1 * 18300, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
additional information
-
activity increases in nearly a linear fashion between pH 6.0 and 9.5
Rattus norvegicus