BRENDA - Enzyme Database show

Enzymatic methylation of arsenic compounds: assay, partial purification, and properties of arsenite methyltransferase and monomethylarsonic acid methyltransferase of rabbit liver

Zakharyan, R.; Wu, Y.; Bogdan, G.M.; Aposhian, H.V.; Chem. Res. Toxicol. 8, 1029-1038 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
dithiothreitol
-
Oryctolagus cuniculus
2.1.1.137
glutathione
-
Oryctolagus cuniculus
2.1.1.137
L-cysteine
-
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.137
HgCl2
-
Oryctolagus cuniculus
2.1.1.137
selenate
more than 1 mM selenate necessary for 50% inhibition
Oryctolagus cuniculus
2.1.1.137
Selenite
50% inhibition with 0.0126 mM selinite
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
60000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Oryctolagus cuniculus
-
S-adenosyl-L-homocysteine + methylarsonate
-
Oryctolagus cuniculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
DEAE cellulose, ammonium sulfate precipitation, Sephadex G-200, Sephadex G-100, 2000fold purification
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.1.137
0.00000167
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
485152
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + methylarsonate
-
485152
Oryctolagus cuniculus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
6.8
-
optimum if enzyme activity is measured in crude extracts
Oryctolagus cuniculus
2.1.1.137
8.2
-
-
Oryctolagus cuniculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
dithiothreitol
-
Oryctolagus cuniculus
2.1.1.137
glutathione
-
Oryctolagus cuniculus
2.1.1.137
L-cysteine
-
Oryctolagus cuniculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.137
HgCl2
-
Oryctolagus cuniculus
2.1.1.137
selenate
more than 1 mM selenate necessary for 50% inhibition
Oryctolagus cuniculus
2.1.1.137
Selenite
50% inhibition with 0.0126 mM selinite
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
60000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Oryctolagus cuniculus
-
S-adenosyl-L-homocysteine + methylarsonate
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
DEAE cellulose, ammonium sulfate precipitation, Sephadex G-200, Sephadex G-100, 2000fold purification
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.1.137
0.00000167
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
485152
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + methylarsonate
-
485152
Oryctolagus cuniculus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
6.8
-
optimum if enzyme activity is measured in crude extracts
Oryctolagus cuniculus
2.1.1.137
8.2
-
-
Oryctolagus cuniculus