BRENDA - Enzyme Database show

Enzymatic methylation of arsenic compounds. VI. Characterization of hamster liver arsenite and methylarsonic acid methyltransferase activities in vitro

Wildfang, E.; Zakharyan, R.A.; Aposhian, H.V.; Toxicol. Appl. Pharmacol. 152, 366-375 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
dithiothreitol
weak activation
Cricetinae
2.1.1.137
glutathione
a 4fold increase in glutathione concentration, 2 to 8 mM, leads to a 2fold increase in enzyme activity, reducing environment is required for enzyme activity
Cricetinae
2.1.1.137
L-cysteine
activates stronger than glutathione
Cricetinae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.002
-
arsenite
-
Cricetinae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Cricetinae
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
46000
-
gel filtration
Cricetinae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cricetinae
-
S-adenosyl-L-homocysteine + methylarsonate
-
Cricetinae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Cricetinae
-
male golden syrian hamster
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
DEAE anion exchange chromatography, Sephadex G-200, High Q anion exchange chromatographie, 40fold purification
Cricetinae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
liver
-
Cricetinae
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.137
0.0000000283
-
-
Cricetinae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
485148
Cricetinae
S-adenosyl-L-homocysteine + methylarsonate
-
485148
Cricetinae
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.6
-
-
Cricetinae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Cricetinae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
dithiothreitol
weak activation
Cricetinae
2.1.1.137
glutathione
a 4fold increase in glutathione concentration, 2 to 8 mM, leads to a 2fold increase in enzyme activity, reducing environment is required for enzyme activity
Cricetinae
2.1.1.137
L-cysteine
activates stronger than glutathione
Cricetinae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.137
S-adenosyl-L-methionine
-
Cricetinae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.002
-
arsenite
-
Cricetinae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.137
cytosol
-
Cricetinae
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
46000
-
gel filtration
Cricetinae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Cricetinae
-
S-adenosyl-L-homocysteine + methylarsonate
-
Cricetinae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
DEAE anion exchange chromatography, Sephadex G-200, High Q anion exchange chromatographie, 40fold purification
Cricetinae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
liver
-
Cricetinae
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.137
0.0000000283
-
-
Cricetinae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
485148
Cricetinae
S-adenosyl-L-homocysteine + methylarsonate
-
485148
Cricetinae
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.137
7.6
-
-
Cricetinae