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Literature summary extracted from

  • Wildfang, E.; Zakharyan, R.A.; Aposhian, H.V.
    Enzymatic methylation of arsenic compounds. VI. Characterization of hamster liver arsenite and methylarsonic acid methyltransferase activities in vitro (1998), Toxicol. Appl. Pharmacol., 152, 366-375.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.137 dithiothreitol weak activation Cricetinae
2.1.1.137 glutathione a 4fold increase in glutathione concentration, 2 to 8 mM, leads to a 2fold increase in enzyme activity, reducing environment is required for enzyme activity Cricetinae
2.1.1.137 L-cysteine activates stronger than glutathione Cricetinae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.137 0.002
-
arsenite
-
Cricetinae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.137 cytosol
-
Cricetinae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.137 46000
-
gel filtration Cricetinae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite Cricetinae
-
S-adenosyl-L-homocysteine + methylarsonate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Cricetinae
-
male golden syrian hamster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 DEAE anion exchange chromatography, Sephadex G-200, High Q anion exchange chromatographie, 40fold purification Cricetinae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.137 liver
-
Cricetinae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.137 0.0000000283
-
-
Cricetinae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
Cricetinae S-adenosyl-L-homocysteine + methylarsonate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.137 7.6
-
-
Cricetinae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.137 S-adenosyl-L-methionine
-
Cricetinae