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Literature summary extracted from

  • Raux, E.; McVeigh, T.; Peters, S.E.; Leustek, T.; Warren, M.J.
    The role of Saccharomyces cerevisiae MET1p and MET8p in sirohaem and cobalamin biosynthesis (1999), Biochem. J., 338, 701-708.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.76 MET8 mutants complemented by Salmonella typhimurium cysG, cloned and expressed in Escherichia coli Saccharomyces cerevisiae
2.1.1.107 EC 2.1.1.107 deficient mutants complemented with cysG from Salmonella typhimurium and Escherichia coli and cobA from Pseudomonas denitrificans, overexpression of yeast gene product in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ Saccharomyces cerevisiae siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Salmonella enterica subsp. enterica serovar Typhimurium
-
 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Escherichia coli
-
 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Pseudomonas denitrificans (nom. rej.)
-
 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Saccharomyces cerevisiae involved in biosynthesis of siroheme 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.76 Saccharomyces cerevisiae
-
 yeast
-
2.1.1.107 Escherichia coli
-
-
-
2.1.1.107 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.107 Saccharomyces cerevisiae
-
-
-
2.1.1.107 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.76
-
 Saccharomyces cerevisiae
2.1.1.107 from recombinant E. coli using His-tag Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+
-
 Saccharomyces cerevisiae sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Saccharomyces cerevisiae sirohydrochlorin + NADH + H+
-
 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Pseudomonas denitrificans (nom. rej.) 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III methylates positions 2 and 7 Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of siroheme Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.76 cobaltochelatase
-
 Saccharomyces cerevisiae
1.3.1.76 Met8p
-
 Saccharomyces cerevisiae