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Literature summary extracted from

  • Reinhard, K.; Matern, U.
    The biosynthesis of phytoalexins in Dianthus caryophyllus L. cell cultures: induction of benzoyl-CoA:anthranilate N-benzoyltransferase activity (1989), Arch. Biochem. Biophys., 275, 295-301.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.144 0.033
-
 anthranilate
-
 Dianthus caryophyllus
2.3.1.144 0.05
-
 benzoyl-CoA
-
 Dianthus caryophyllus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.144 benzoyl-CoA + anthranilate Dianthus caryophyllus cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors with 73%, 70% and 65% efficiency respectively, enzyme is involved in the biosynthesis of phytoalexins, 4-hydroxyanthranilate is the most probable substrate in vivo CoA + N-benzoylanthranilate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.105 Dianthus caryophyllus
-
 enzyme activity is induced by treatment of the cells with elicitor
-
2.3.1.144 Dianthus caryophyllus
-
 enzyme is induced by addition of crude elicitor from the cell walls of Phythophthora megasperma f. sp. glycinea or by commercial yeast extract
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.144 ammonium sulfate, Q-Sepharose, partially purified Dianthus caryophyllus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.105 cell culture
-
 Dianthus caryophyllus
-
2.3.1.144 callus cell culture Dianthus caryophyllus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.105 S-adenosyl-L-methionine + N-benzoyl-4-hydroxyanthranilate
-
 Dianthus caryophyllus S-adenosyl-L-homocysteine + N-benzoyl-4-methoxyanthranilate
-
 ?
2.3.1.144 benzoyl-CoA + 3-hydroxyanthranilate 20% of activity with anthranilate Dianthus caryophyllus CoA + N-benzoyl-3-hydroxyanthranilate
-
 ?
2.3.1.144 benzoyl-CoA + 4-hydroxyanthranilate 10% of activity with anthranilate Dianthus caryophyllus CoA + N-benzoyl-4-hydroxyanthranilate
-
 ?
2.3.1.144 benzoyl-CoA + anthranilate cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors Dianthus caryophyllus CoA + N-benzoylanthranilate
-
 ?
2.3.1.144 benzoyl-CoA + anthranilate cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors with 73%, 70% and 65% efficiency respectively, enzyme is involved in the biosynthesis of phytoalexins, 4-hydroxyanthranilate is the most probable substrate in vivo Dianthus caryophyllus CoA + N-benzoylanthranilate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.144 20
-
-
 Dianthus caryophyllus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.144 40
-
 crude extract, 5 min, pH 7.5, significant loss of activity Dianthus caryophyllus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.144 additional information
-
 true pH-optimum may be closer to the amino-pK of anthranilate at 4.95 Dianthus caryophyllus
2.3.1.144 6.8 7 crude extract, assay at pH 7.0 Dianthus caryophyllus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.144 5.5 7.8
-
 Dianthus caryophyllus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.144 6
-
 crude extract, 4°C, almost all activity irreversibly lost within less than 2 h Dianthus caryophyllus
2.3.1.144 7
-
 very labile at pH below Dianthus caryophyllus