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Literature summary extracted from

  • Parvathi, K.; Chen, F.; Guo, D.; Blount, J.W.; Dixon, R.A.
    Substrate preferences of O-methyltransferases in alfalfa suggest new pathways for 3-O-methylation of monolignols (2001), Plant J., 25, 193-202.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expression in Escherichia coli BL21 Medicago sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.0018
-
 5-hydroxyconiferaldehyde recombinant enzyme Medicago sativa
2.1.1.68 0.0038
-
 5-hydroxyconiferyl alcohol recombinant enzyme Medicago sativa
2.1.1.68 0.0068
-
 5-hydroxyferulic acid recombinant enzyme Medicago sativa
2.1.1.68 0.0069
-
 caffeoyl aldehyde recombinant enzyme Medicago sativa
2.1.1.68 0.012
-
 caffeoyl alcohol recombinant enzyme Medicago sativa
2.1.1.68 0.06
-
 caffeic acid recombinant enzyme Medicago sativa
2.1.1.104 0.0043
-
 caffeoyl-CoA recombinant enzyme Medicago sativa
2.1.1.104 0.0062
-
 5-hydroxyferuoyl-CoA recombinant enzyme Medicago sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 43000
-
 SDS-PAGE, recombinant enzyme Medicago sativa
2.1.1.104 30000
-
 SDS-PAGE Medicago sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol Medicago sativa alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde Medicago sativa alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Medicago sativa
-
 strain cv Apollo
-
2.1.1.68 Medicago sativa
-
 transgenic line with downregulated caffeate O-methyltransferase activity, cv Regen SY
-
2.1.1.104 Medicago sativa
-
 alfalfa
-
2.1.1.104 Medicago sativa
-
 cv Apollo for non-transgenic and cv Regen SY for transgenic line with downregulated CCoAOMT
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 stem first to tenth stem internodes of non-transgenic alfalfa Medicago sativa
-
2.1.1.104 stem first to the tenth stem internodes Medicago sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapaldehyde
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
 Medicago sativa S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Medicago sativa S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol Medicago sativa ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Medicago sativa S-adenosyl-L-homocysteine + coniferyl aldehyde
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol Medicago sativa ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapaldehyde
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyferuoyl-CoA
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Medicago sativa S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 COMT
-
 Medicago sativa
2.1.1.104 CCoAOMT
-
 Medicago sativa