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Literature summary extracted from

  • Charron, J.B.F.; Breton, G.; Danyluk, J.; Muzac, I.; Ibrahim, R.K.; Sarhan, F.
    Molecular and biochemical characterization of a cold-regulated phosphoethanolamine N-methyltransferase from wheat (2002), Plant Physiol., 129, 363-373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 in Escherichia coli Triticum aestivum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.103 additional information several point mutations and N- and C-terminal domains investigated, N-terminal domain catalyzes all three activities, regulatory role for C-terminal domain suggested Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.056
-
 S-adenosyl-L-methionine
-
 Triticum aestivum
2.1.1.103 0.065
-
 Ethanolamine phosphate
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.103 57000
-
 calculated from DNA sequence Triticum aestivum
2.1.1.103 65000
-
 SDS-PAGE, enzyme from E. coli, contains of 57 kD enzyme protein + 8 kD His-tag Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Triticum aestivum involved in synthesis of phosphocholine and phosphatidylcholine S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Triticum aestivum Q8VYX1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 from E. coli, His-tag used for affinity chromatography Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate
-
 Triticum aestivum S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Triticum aestivum S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in synthesis of phosphocholine and phosphatidylcholine Triticum aestivum S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine
-
 Triticum aestivum S-adenosyl-L-homocysteine + phosphocholine
-
 ?