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Literature summary extracted from

  • Datko, A.H.; Mudd, H.S.
    Enzymes of phosphatidylcholine synthesis in lemna, soybean, and carrot (1988), Plant Physiol., 88, 1338-1348.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.103 EDTA removal of EDTA leads to 60% loss of activity Lemna aequinoctialis
2.1.1.103 glutathione removal of glutathione leads to almost complete loss of activity Lemna aequinoctialis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Glycine max involved in synthesis of phosphocholine and phosphatidylcholine S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Daucus carota involved in synthesis of phosphocholine and phosphatidylcholine S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Lemna aequinoctialis involved in synthesis of phosphocholine and phosphatidylcholine S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.7.7.57 additional information Glycine max involved in biosynthesis of phosphatidylcholine ?
-
 ?
2.7.7.57 additional information Daucus carota involved in biosynthesis of phosphatidylcholine ?
-
 ?
2.7.7.57 additional information Lemna aequinoctialis involved in biosynthesis of phosphatidylcholine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Daucus carota
-
 carrot
-
2.1.1.103 Glycine max
-
 soybean
-
2.1.1.103 Lemna aequinoctialis
-
 duck weed
-
2.7.7.57 Daucus carota
-
 cv. Danvers
-
2.7.7.57 Glycine max
-
 cv. Peking
-
2.7.7.57 Lemna aequinoctialis
-
 Hegelm. 6746
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.57 partial Glycine max
2.7.7.57 partial Daucus carota
2.7.7.57 partial Lemna aequinoctialis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.57 cell suspension culture
-
 Glycine max
-
2.7.7.57 cell suspension culture
-
 Daucus carota
-
2.7.7.57 plant homogenized Lemna aequinoctialis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 additional information no activity with phosphomethylethanolamine and phosphodimethylethanolamine Glycine max ?
-
 ?
2.1.1.103 additional information no activity with ethanolamine, methylethanolamine and dimethylethanolamine Lemna aequinoctialis ?
-
 ?
2.1.1.103 S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate
-
 Daucus carota S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate
-
 Lemna aequinoctialis S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Glycine max S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Daucus carota S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Lemna aequinoctialis S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in synthesis of phosphocholine and phosphatidylcholine Glycine max S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in synthesis of phosphocholine and phosphatidylcholine Daucus carota S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in synthesis of phosphocholine and phosphatidylcholine Lemna aequinoctialis S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine
-
 Daucus carota S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine
-
 Lemna aequinoctialis S-adenosyl-L-homocysteine + phosphocholine
-
 ?
2.7.7.57 CTP + choline phosphate best substrate Glycine max CDP-choline + diphosphate CDP-choline demonstrated in crude extract ?
2.7.7.57 CTP + choline phosphate best substrate Daucus carota CDP-choline + diphosphate CDP-choline demonstrated in crude extract ?
2.7.7.57 CTP + choline phosphate best substrate Lemna aequinoctialis CDP-choline + diphosphate CDP-choline demonstrated in crude extract ?
2.7.7.57 CTP + N,N-dimethylethanolamine phosphate
-
 Daucus carota CDP-N,N-dimethylethanolamine + diphosphate CDP-N,N-dimethylethanolamine demonstrated in crude extract ?
2.7.7.57 CTP + N,N-dimethylethanolamine phosphate
-
 Lemna aequinoctialis CDP-N,N-dimethylethanolamine + diphosphate CDP-N,N-dimethylethanolamine demonstrated in crude extract ?
2.7.7.57 CTP + N-methylethanolamine phosphate
-
 Daucus carota CDP-N-methylethanolamine + diphosphate CDP-N-ethanolamine demonstrated in crude extract ?
2.7.7.57 CTP + N-methylethanolamine phosphate
-
 Lemna aequinoctialis CDP-N-methylethanolamine + diphosphate CDP-N-ethanolamine demonstrated in crude extract ?
2.7.7.57 additional information involved in biosynthesis of phosphatidylcholine Glycine max ?
-
 ?
2.7.7.57 additional information involved in biosynthesis of phosphatidylcholine Daucus carota ?
-
 ?
2.7.7.57 additional information involved in biosynthesis of phosphatidylcholine Lemna aequinoctialis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.57 30
-
 assay at Glycine max
2.7.7.57 30
-
 assay at Daucus carota
2.7.7.57 30
-
 assay at Lemna aequinoctialis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.57 6.4 8.5 activity rates with different substrates, overview Glycine max
2.7.7.57 6.4 8.5 activity rates with different substrates, overview Daucus carota
2.7.7.57 6.4 8.5 activity rates with different substrates, overview Lemna aequinoctialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.103 7.8
-
-
 Lemna aequinoctialis
2.7.7.57 6.4
-
 assay at Glycine max
2.7.7.57 6.4
-
 assay at Daucus carota
2.7.7.57 6.4
-
 assay at Lemna aequinoctialis
2.7.7.57 7.4
-
 substrate choline phosphate Glycine max
2.7.7.57 7.4
-
 substrate choline phosphate Daucus carota
2.7.7.57 7.4
-
 substrate choline phosphate Lemna aequinoctialis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.103 6.8 8.3
-
 Lemna aequinoctialis