EC Number | General Stability | Organism |
---|---|---|
1.5.1.34 | not stable during gel filtration | Crithidia fasciculata |
1.5.1.34 | unstable to (NH4)2SO4 precipitation | Crithidia fasciculata |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.34 | aminopterin | 1 mM, 50% inhibition | Crithidia fasciculata | |
1.5.1.34 | methopterin | 1 mM, 10% inhibition | Crithidia fasciculata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.34 | 0.0028 | - |
NADH | - |
Crithidia fasciculata | |
1.5.1.34 | 0.0088 | - |
NADPH | - |
Crithidia fasciculata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 55000 | - |
gel filtration | Crithidia fasciculata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.34 | Crithidia fasciculata | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.34 | -80°C, 40 mM potassium phosphate buffer, pH 6.8, stable for at least a week | Crithidia fasciculata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.34 | quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | more specific for NADH, 50% activity with NADPH, no activity with biopterin and 7,8-dihydrobiopterin | Crithidia fasciculata | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
1.5.1.34 | quinonoid dihydrobiopterin + NADH + H+ | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrobiopterin + NAD+ | - |
r |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 6.5 | 8 | unstable below and above | Crithidia fasciculata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.34 | NADH | utilizes both NADH and NADPH | Crithidia fasciculata | |
1.5.1.34 | NADH | enzyme prefers NADH | Crithidia fasciculata | |
1.5.1.34 | NADPH | utilizes both NADH and NADPH | Crithidia fasciculata | |
1.5.1.34 | NADPH | NADH is preferred | Crithidia fasciculata |