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Literature summary extracted from

  • Suye, S.
    Purification and properties of alcohol oxidase from Candida methanosorbosa M-2003 (1997), Curr. Microbiol., 34, 374-377.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.13 7.7 mM NaN3, 30°C, enzyme retains activity for at least 35 days, inactivated completely after 10 days without NaN3 [Candida] methanosorbosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 Cu2+ 1 mM CuSO4, 30 min, strong [Candida] methanosorbosa
1.1.3.13 Hg2+ 1 mM HgCl2, 30 min, strong [Candida] methanosorbosa
1.1.3.13 hydroquinone 1 mM, 30 min, strong [Candida] methanosorbosa
1.1.3.13 NEM 1 mM, 30 min, strong [Candida] methanosorbosa
1.1.3.13 PCMB 1 mM, 30 min, strong [Candida] methanosorbosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 2.43
-
 methanol
-
 [Candida] methanosorbosa
1.1.3.13 8.23
-
 ethanol
-
 [Candida] methanosorbosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 80000
-
 8 * 80000, SDS-PAGE [Candida] methanosorbosa
1.1.3.13 620000
-
 gel filtration [Candida] methanosorbosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 [Candida] methanosorbosa
-
 M-2003
-
1.1.3.13 [Candida] methanosorbosa M-2003
-
 M-2003
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 [Candida] methanosorbosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 culture medium
-
 [Candida] methanosorbosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 4.11
-
-
 [Candida] methanosorbosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-octanol + O2 2.4% of the activity with methanol [Candida] methanosorbosa n-octanal + H2O2
-
 ?
1.1.3.13 1-pentanol + O2 33.5% of the activity with methanol [Candida] methanosorbosa n-pentanal + H2O2
-
 ?
1.1.3.13 1-pentanol + O2 33.5% of the activity with methanol [Candida] methanosorbosa M-2003 n-pentanal + H2O2
-
 ?
1.1.3.13 2-butanol + O2 3.5% of the activity with methanol [Candida] methanosorbosa 2-butanone + H2O2
-
 ?
1.1.3.13 2-butanol + O2 3.5% of the activity with methanol [Candida] methanosorbosa M-2003 2-butanone + H2O2
-
 ?
1.1.3.13 benzyl alcohol + O2 21.3% of the activity with methanol [Candida] methanosorbosa benzaldehyde + H2O2
-
 ?
1.1.3.13 benzyl alcohol + O2 21.3% of the activity with methanol [Candida] methanosorbosa M-2003 benzaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 97.8% of the activity with methanol [Candida] methanosorbosa acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 97.8% of the activity with methanol [Candida] methanosorbosa M-2003 acetaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 [Candida] methanosorbosa formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 [Candida] methanosorbosa M-2003 formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2 52.2% of the activity with methanol [Candida] methanosorbosa n-butanal + H2O2
-
 ?
1.1.3.13 n-hexanol + O2 6.3% of the activity with methanol [Candida] methanosorbosa n-hexanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2 77.5% of the activity with methanol [Candida] methanosorbosa n-propanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 octamer 8 * 80000, SDS-PAGE [Candida] methanosorbosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 50
-
-
 [Candida] methanosorbosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 30
-
 pH 6.0-8.5. 60 min, stable [Candida] methanosorbosa
1.1.3.13 50
-
 pH 7.5, 15 min, stable below [Candida] methanosorbosa
1.1.3.13 55
-
 15 min, 34% loss of activity [Candida] methanosorbosa
1.1.3.13 65
-
 15 min, complete inactivation above [Candida] methanosorbosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 6 9
-
 [Candida] methanosorbosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD
-
 [Candida] methanosorbosa