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Literature summary extracted from

  • Nishida, A.; Eriksson, K.E.
    Formation, purification, and partial characterization of methanol oxidase, a H2O2-producing enzyme in Phanerochaete chrysosporium (1987), Biotechnol. Appl. Biochem., 9, 325-338.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.785
-
 methanol
-
 Phanerodontia chrysosporium
1.1.3.13 1.97
-
 ethanol
-
 Phanerodontia chrysosporium
1.1.3.13 13.8
-
 n-Propanol
-
 Phanerodontia chrysosporium
1.1.3.13 35.9
-
 n-butanol
-
 Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 310000
-
 gel filtration Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 methanol + O2 Phanerodontia chrysosporium key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 Phanerodontia chrysosporium K-3 key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Phanerodontia chrysosporium
-
-
-
1.1.3.13 Phanerodontia chrysosporium K-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Phanerodontia chrysosporium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 mycelium
-
 Phanerodontia chrysosporium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 16.5
-
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 ethanol + O2 92% of the activity with methanol Phanerodontia chrysosporium acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 92% of the activity with methanol Phanerodontia chrysosporium K-3 acetaldehyde + H2O2
-
 ?
1.1.3.13 ethylene glycol + O2 1.7% of the activity with methanol Phanerodontia chrysosporium ?
-
 ?
1.1.3.13 ethylene glycol mono-methylether + O2 10% of the activity with methanol Phanerodontia chrysosporium methoxyacetaldehyde + H2O2
-
 ?
1.1.3.13 isopropanol + O2
-
 Phanerodontia chrysosporium 2-propanone + H2O2
-
 ?
1.1.3.13 isopropanol + O2
-
 Phanerodontia chrysosporium K-3 2-propanone + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Phanerodontia chrysosporium formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Phanerodontia chrysosporium formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Phanerodontia chrysosporium K-3 formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Phanerodontia chrysosporium K-3 formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2 10.6% of the activity with methanol Phanerodontia chrysosporium n-butanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2
-
 Phanerodontia chrysosporium n-propanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2
-
 Phanerodontia chrysosporium K-3 n-propanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 tetramer
-
 Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 30
-
 15 min, about 30% loss of activity Phanerodontia chrysosporium
1.1.3.13 40
-
 15 min, about 85% loss of activity Phanerodontia chrysosporium
1.1.3.13 50
-
 15 min, complete inactivation Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 6 10.5
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.13 5.5 11 pH 5.5: 6% of maximal activity, pH 11.0: 12% of maximal activity Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD 5.62 mol per mol enzyme Phanerodontia chrysosporium