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Literature summary extracted from

  • Janssen, F.W.; Ruelius, H.W.
    Alcohol oxidase, a flavoprotein from several Basidiomycetes species. Crystallization by fractional precipitation with polyethylene glycol (1968), Biochim. Biophys. Acta, 151, 330-342.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.13 analysis enzyme may be useful for the colorimetric determination of methanol, ethanol, or other alcohols Basidiomycota

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.13
-
 Basidiomycota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 1.52
-
 methanol
-
 Basidiomycota
1.1.3.13 10
-
 ethanol
-
 Basidiomycota
1.1.3.13 54.6
-
 n-Propanol
-
 Basidiomycota
1.1.3.13 133
-
 n-butanol
-
 Basidiomycota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 300000
-
 above, gel filtration Basidiomycota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 methanol + O2 Basidiomycota key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Basidiomycota
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.13 no glycoprotein
-
 Basidiomycota

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Basidiomycota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 mycelium
-
 Basidiomycota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 additional information
-
-
 Basidiomycota

Storage Stability

EC Number Storage Stability Organism
1.1.3.13 crystalline, stable for at least 5 months in 0.05 M phosphate buffer, pH 7.7 Basidiomycota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-pentanol + O2 1% of the activity with methanol Basidiomycota n-pentanal + H2O2
-
 ?
1.1.3.13 2-propen-1-ol + O2 17% of the activity with methanol Basidiomycota 2-propen-1-al + H2O2
-
 ?
1.1.3.13 2-propyn-1-ol + O2 45% of the activity with methanol Basidiomycota propyn-1-al + H2O2
-
 ?
1.1.3.13 bromoethanol + O2 4% of the activity with methanol Basidiomycota bromoethanal + H2O2
-
 ?
1.1.3.13 chloroethanol + O2 6% of the activity with methanol Basidiomycota chloroethanal + H2O2
-
 ?
1.1.3.13 chloroethanol + O2 6% of the activity with Basidiomycota chloroethanal + H2O2
-
 ?
1.1.3.13 ethanol + O2 28% of the activity with methanol Basidiomycota acetaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Basidiomycota formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Basidiomycota formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2 2.1% of the activity with methanol Basidiomycota n-butanal + H2O2
-
 ?
1.1.3.13 n-butanol + O2 2.1%, of the activity with methanol Basidiomycota n-butanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2 5.3% of the activity with methanol Basidiomycota n-propanal + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 25
-
 pH 5.0, 3 h, complete inactivation at pH 5.0 after 3 h Basidiomycota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 6.3 9
-
 Basidiomycota
1.1.3.13 6.5 9
-
 Basidiomycota

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 5
-
 25°C, 3 h, complete inactivation Basidiomycota
1.1.3.13 6.5
-
 rapid inactivation below Basidiomycota
1.1.3.13 7 9 25°C, 3 h, stable Basidiomycota

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD
-
 Basidiomycota