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Literature summary extracted from

  • Leach F.R.
    ATP determination with firefly luciferase (1981), J. Appl. Biochem., 3, 473-517.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.12.7 bovine serum albumin stabilizes Photinus pyralis
1.13.12.7 EDTA stabilizes Photinus pyralis
1.13.12.7 EDTA, 2-mercaptoethanol, dithiothreitol and bovine serum albumin used as stabilizers Photinus pyralis
1.13.12.7 stabilized by immobilization on polysaccharide carriers Photinus pyralis
1.13.12.7 stabilized by immobilization on polysaccharide carriers Luciola mingrelica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 3',5'-cyclic AMP competitive inhibitor Photinus pyralis
1.13.12.7 5-anilino-1-naphthalene sulfonate competitive inhibitor Photinus pyralis
1.13.12.7 6-toluidino-2-naphthalene sulfonate competitive inhibitor Photinus pyralis
1.13.12.7 arsenate
-
 Photinus pyralis
1.13.12.7 ATP
-
 Photinus pyralis
1.13.12.7 dehydroluciferin competitive inhibitor Photinus pyralis
1.13.12.7 ethanol
-
 Photinus pyralis
1.13.12.7 ethyl-2-benzothiazole sulfonate competitive inhibitor Photinus pyralis
1.13.12.7 L-1-tosylamido-2-phenethyl chlorometyl ketone competitive inhibitor with respect to luciferin, noncompetitive with respect to ATP Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.5
-
 ATP crude extract Photinus pyralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 50000 52000 high speed equilibrium sedimentation after 5 M guanidine hydrochloride Photinus pyralis
1.13.12.7 89000 91000 low speed equilibrium sedimentation Photinus pyralis
1.13.12.7 92000 95000 high speed equilibrium sedimentation Photinus pyralis
1.13.12.7 100000
-
 from sedimentation velocity of the crystalline form Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Luciola mingrelica
-
-
-
1.13.12.7 Photinus pyralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 chromatography on DEAE-cellulose Luciola mingrelica
1.13.12.7 several chromatographic and non chromatographic methods Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.7 dimer 2 * 48000-53000, various methods Photinus pyralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 23
-
 purified Photinus pyralis
1.13.12.7 25
-
-
 Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 additional information
-
 pH 7.8 crude extract Photinus pyralis
1.13.12.7 7.8
-
 purified enzyme Photinus pyralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.7 7 8
-
 Photinus pyralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.7 0.001
-
 dehydroluciferin
-
 Photinus pyralis
1.13.12.7 0.0059
-
 6-toluidino-2-naphthalene sulfonate
-
 Photinus pyralis
1.13.12.7 0.23
-
 5-anilino-1-naphthalene sulfonate
-
 Photinus pyralis
1.13.12.7 0.25
-
 L-tosylamido-2-phenethyl chloromethyl ketone
-
 Photinus pyralis