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Literature summary extracted from

  • Takita, T.; Ohkubo, Y.; Shima, H.; Muto, T.; Shimizu, N.; Sukata, H.; Ito, Y.; Saito, Y.; Inouye, K.; Hiromi, K.; Tonomura, B.
    Lysyl-tRNA synthetase from Bacillus stearothermophilus. Purification and fluorometric and kinetic analysis of the binding of substrates, L-lysine and ATP (1996), J. Biochem., 119, 680-689.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.6 6-amino-n-hexanoic acid ATP-diphosphate exchange reaction Geobacillus stearothermophilus
6.1.1.6 adenosine
-
 Geobacillus stearothermophilus
6.1.1.6 ATP substrate inhibition in the aminoacylation reaction above 0.4 mM, no inhibition in the ATP-diphosphate exchange reaction Geobacillus stearothermophilus
6.1.1.6 cadaverine ATP-diphosphate exchange reaction Geobacillus stearothermophilus
6.1.1.6 L-Lysine amide ATP-diphosphate exchange reaction Geobacillus stearothermophilus
6.1.1.6 L-Lysine hydroxamate ATP-diphosphate exchange reaction Geobacillus stearothermophilus
6.1.1.6 Nepsilon-Acetyl-L-lysine ATP-diphosphate exchange reaction Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.6 0.0164
-
 L-Lys aminoacylation Geobacillus stearothermophilus
6.1.1.6 0.0232
-
 ATP aminoacylation Geobacillus stearothermophilus
6.1.1.6 0.0236
-
 L-Lys ATP-diphosphate exchange Geobacillus stearothermophilus
6.1.1.6 0.0651
-
 ATP ATP-diphosphate exchange Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.6 57700
-
 2 * 57700, homodimer, SDS-PAGE, amino acid composition Geobacillus stearothermophilus
6.1.1.6 120000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Geobacillus stearothermophilus
-
 NCA1503
-
6.1.1.6 Geobacillus stearothermophilus NCA1503
-
 NCA1503
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.6
-
 Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.6 ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys sequential ordered mechanism Geobacillus stearothermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.6 1.79
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys
-
 Geobacillus stearothermophilus AMP + diphosphate + L-lysyl-tRNALys
-
 r
6.1.1.6 ATP + L-lysine + tRNALys
-
 Geobacillus stearothermophilus NCA1503 AMP + diphosphate + L-lysyl-tRNALys
-
 r
6.1.1.6 ATP + lysine + tRNALys
-
 Geobacillus stearothermophilus AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + lysine + tRNALys
-
 Geobacillus stearothermophilus NCA1503 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 additional information lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate Geobacillus stearothermophilus ?
-
 ?
6.1.1.6 additional information L-lysine-dependent synthesis of 5',5'-diadenosine tetraphosphate (Ap4A) Geobacillus stearothermophilus ?
-
 ?
6.1.1.6 additional information lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate Geobacillus stearothermophilus NCA1503 ?
-
 ?
6.1.1.6 additional information L-lysine-dependent synthesis of 5',5'-diadenosine tetraphosphate (Ap4A) Geobacillus stearothermophilus NCA1503 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.6 dimer 2 * 57700, homodimer, SDS-PAGE, amino acid composition Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.6 50
-
 aminoacylation reaction and ATP-diphosphate exchange, pH 8 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.6 additional information
-
 inactive at 70°C Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.6 50
-
 stable below, pH 8.0, 5 h Geobacillus stearothermophilus
6.1.1.6 60
-
 after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.6 3.2
-
 L-lysine
-
 Geobacillus stearothermophilus
6.1.1.6 40.9
-
 ATP ATP-diphosphate exchange Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.6 8.3
-
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.1.1.6 8
-
 5 h, stable below 50°C Geobacillus stearothermophilus