BRENDA - Enzyme Database

Purification and properties of phosphatidylserine decarboxylase from Escherichia coli

Dowhan, W.; Wickner, W.T.; Kennedy, E.P.; J. Biol. Chem. 249, 3079-3084 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.65
nonionic detergents
e.g. Triton X-100, absolute requirement
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.65
ionic detergents
e.g., Barlox-12, SDS
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.65
membrane
bound to
Escherichia coli
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.65
36500
-
x * 36500, SDS-PAGE
Escherichia coli
4.1.1.65
60000
170000
sucrose density gradient centrifugation, in presence of Triton
Escherichia coli
4.1.1.65
170000
-
sucrose density gradient centrifugation in absence of Triton
Escherichia coli
4.1.1.65
400000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.65
phosphatidyl-L-serine
Escherichia coli
the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.65
Escherichia coli
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.65
49
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.65
-25°C, pH 6.0-7.8, 10% glycerol, 0.1% Triton X-100, stable for 1.5 years. 0°C, stable for several months. Room temperature, stable for at least 1 week
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.65
Phosphatidyl-L-serine
-
4764
Escherichia coli
Phosphatidylethanolamine + CO2
-
4764
Escherichia coli
-
4.1.1.65
phosphatidyl-L-serine
the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine
4764
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.65
?
x * 36500, SDS-PAGE
Escherichia coli
4.1.1.65
dimer
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.65
6.5
7.5
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.65
nonionic detergents
e.g. Triton X-100, absolute requirement
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.65
ionic detergents
e.g., Barlox-12, SDS
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.65
membrane
bound to
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.65
36500
-
x * 36500, SDS-PAGE
Escherichia coli
4.1.1.65
60000
170000
sucrose density gradient centrifugation, in presence of Triton
Escherichia coli
4.1.1.65
170000
-
sucrose density gradient centrifugation in absence of Triton
Escherichia coli
4.1.1.65
400000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.65
phosphatidyl-L-serine
Escherichia coli
the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.65
49
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.65
-25°C, pH 6.0-7.8, 10% glycerol, 0.1% Triton X-100, stable for 1.5 years. 0°C, stable for several months. Room temperature, stable for at least 1 week
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.65
Phosphatidyl-L-serine
-
4764
Escherichia coli
Phosphatidylethanolamine + CO2
-
4764
Escherichia coli
-
4.1.1.65
phosphatidyl-L-serine
the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine
4764
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.65
?
x * 36500, SDS-PAGE
Escherichia coli
4.1.1.65
dimer
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.65
6.5
7.5
-
Escherichia coli