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Literature summary extracted from

  • He, Z.; Wiegel, J.
    Purification and characterization of an oxygen-sensitive reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum (1996), J. Bacteriol., 178, 3539-3543.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.63 2,3,4-Trihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 3,4,5-Trihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 Avidin
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 HgCl2
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 hydroxylamine
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 NaBH4
-
 Sedimentibacter hydroxybenzoicus
4.1.1.63 ZnSO4
-
 Sedimentibacter hydroxybenzoicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.63 0.59
-
 3,4-dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.63 57000
-
 5 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus
4.1.1.63 270000
-
 non-denaturing gradient PAGE Sedimentibacter hydroxybenzoicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.63 Sedimentibacter hydroxybenzoicus
-
 Clostridium hydroxybenzoicum, JW/Z-1T
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.1.63 oxygen sensitive. 50% loss of activity after 10 min at 25°C, when exposed to air Sedimentibacter hydroxybenzoicus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.63
-
 Sedimentibacter hydroxybenzoicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.63 20.6
-
-
 Sedimentibacter hydroxybenzoicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.63 3,4-Dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus Catechol + CO2
-
 ?
4.1.1.63 Catechol + CO2
-
 Sedimentibacter hydroxybenzoicus 3,4-Dihydroxybenzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.63 pentamer 5 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.63 50
-
-
 Sedimentibacter hydroxybenzoicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.63 11 50 pH 7.0, relatively stable Sedimentibacter hydroxybenzoicus
4.1.1.63 25
-
 pH 6.5-9.5, 2 h, stable Sedimentibacter hydroxybenzoicus
4.1.1.63 55
-
 half-life: 1.5 h Sedimentibacter hydroxybenzoicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.63 90
-
 3,4-dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.63 7
-
 under anaerobic conditions, both directions Sedimentibacter hydroxybenzoicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.63 6.5 9.5 25°C, 2 h, stable Sedimentibacter hydroxybenzoicus