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Literature summary extracted from

  • He, Z.; Wiegel, J.
    Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum (1995), Eur. J. Biochem., 229, 77-82.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.61 2,3,4-Trihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 2,4,6-Trihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 2,4-Dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 3,4,5-Trihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 NH4+ decreases carboxylation of 3,4-dihydroxybenzoate by about 20% Sedimentibacter hydroxybenzoicus
4.1.1.61 Rb+ decreases carboxylation of 3,4-dihydroxybenzoate by about 20% Sedimentibacter hydroxybenzoicus
4.1.1.61 Zn2+ destroys carboxylation activity towards 4-hydroxybenzoate and 3,4-dihydroxybenzoate Sedimentibacter hydroxybenzoicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.61 0.4
-
 4-hydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 1.2
-
 3,4-dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.61 Mn2+ minor stimulation of carboxylation of 4-hydroxybenzoate Sedimentibacter hydroxybenzoicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.61 57000
-
 6 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus
4.1.1.61 350000
-
 gradient PAGE, gel filtration Sedimentibacter hydroxybenzoicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.61 Sedimentibacter hydroxybenzoicus
-
 Clostridium hydroxybenzoicum, strain JW/Z-1T
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.1.61 oxygen-sensitive, 50% loss of activity when exposed to air at 5°C for 2 h Sedimentibacter hydroxybenzoicus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.61
-
 Sedimentibacter hydroxybenzoicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.61 8.29
-
-
 Sedimentibacter hydroxybenzoicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.61 3,4-Dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus Catechol + CO2
-
 ?
4.1.1.61 4-Hydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus Phenol + CO2
-
 ?
4.1.1.61 Catechol + CO2
-
 Sedimentibacter hydroxybenzoicus 3,4-Dihydroxybenzoate
-
 ?
4.1.1.61 Phenol + CO2
-
 Sedimentibacter hydroxybenzoicus 4-Hydroxybenzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.61 hexamer 6 * 57000, SDS-PAGE Sedimentibacter hydroxybenzoicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.61 50
-
-
 Sedimentibacter hydroxybenzoicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.61 11 40 pH 6.0, stable for more than 24 h Sedimentibacter hydroxybenzoicus
4.1.1.61 50
-
 half-life: 12 h Sedimentibacter hydroxybenzoicus
4.1.1.61 56
-
 half-life: 15 min Sedimentibacter hydroxybenzoicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.61 18.3
-
 3,4-dihydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus
4.1.1.61 55
-
 4-hydroxybenzoate
-
 Sedimentibacter hydroxybenzoicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.61 5.6 6.2
-
 Sedimentibacter hydroxybenzoicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.61 5.5 7.3 stable for 2 h Sedimentibacter hydroxybenzoicus
4.1.1.61 6
-
 11-40°C, stable for more than 24 h Sedimentibacter hydroxybenzoicus