Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cacciapuoti, G.; Porcelli, M.; de Rosa, M.; Gambacorta, A.; Bertoldo, C.; Zappia, V.
    S-Adenosylmethionine decarboxylase from the thermophilic archaebacterium Sulfolobus solfataricus (1991), Eur. J. Biochem., 199, 395-400.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.50 putrescine no requirement Saccharolobus solfataricus
4.1.1.50 pyruvate covalently linked prosthetic group Saccharolobus solfataricus
4.1.1.50 pyruvate contains approximately 0.8 mol pyruvate per mol of enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.50 methylglyoxal bis(guanylhydrazone) competitive Saccharolobus solfataricus
4.1.1.50 NaCNBH3 in presence of substrate or product Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.96
-
 S-adenosylmethionine
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.50 additional information no requirement for a divalent metal ion Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.50 32000
-
 1 * 32000, SDS-PAGE Saccharolobus solfataricus
4.1.1.50 36000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine Saccharolobus solfataricus major regulatory enzyme in polyamine biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.50
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.50 0.012
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Saccharolobus solfataricus (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?
4.1.1.50 S-adenosyl-L-methionine major regulatory enzyme in polyamine biosynthesis Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 monomer 1 * 32000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.50 75
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.50 50 90 50°C: about 45% of maximal activity, 90°C: about 60% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.50 50
-
 16 h, stable Saccharolobus solfataricus
4.1.1.50 70
-
 16h, 20% loss of activity Saccharolobus solfataricus
4.1.1.50 100
-
 1 h, 80% loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.50 7.4
-
-
 Saccharolobus solfataricus