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Literature summary extracted from

  • Phalip, V.; Monnet, C.; Schmitt, P.; Renault, P.; Godon, J.J.; Divies, C.
    Purification and properties of the alpha-acetolactate decarboxylase from Lactococcus lactis subsp. lactis NCDO 2118 (1994), FEBS Lett., 351, 95-99.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.5 overexpression in Escherichia coli Lactococcus lactis

General Stability

EC Number General Stability Organism
4.1.1.5 very unstable, especially when diluted, at 0.05 mg/ml the activity is totally lost within 24 h at 4°C. Addition of bovine serum albumin, 5 mg/ml, allows the preservation of the activity for at least 2 weeks Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.5 EDTA 2 mM Mn2+ restores 82% of the activity, 2 mM Zn2+ restores 69% of the initial activity Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.5 10
-
 2-Hydroxy-2-methyl-3-oxobutanoate in presence of Leu Lactococcus lactis
4.1.1.5 75
-
 2-Hydroxy-2-methyl-3-oxobutanoate in absence of Leu Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.5 26500
-
 6 * 26500, SDS-PAGE Lactococcus lactis
4.1.1.5 150000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate Lactococcus lactis activation by Leu, Val and Ile ?
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate Lactococcus lactis NCDO 2118 activation by Leu, Val and Ile ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.5 Lactococcus lactis
-
-
-
4.1.1.5 Lactococcus lactis
-
 subsp. lactis
-
4.1.1.5 Lactococcus lactis NCDO 2118
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.5
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.5 454.3
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lactococcus lactis (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lactococcus lactis NCDO 2118 (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate activation by Leu, Val and Ile Lactococcus lactis ?
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate activation by Leu, Val and Ile Lactococcus lactis NCDO 2118 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.5 hexamer 6 * 26500, SDS-PAGE Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.5 6
-
-
 Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.5 5.4 6.9 75% of maximal activity at pH 5.4 and at pH 6.9 Lactococcus lactis