Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshizaki, F.; Imahori, K.
    Properties of phosphoenolpyruvate carboxykinase of an extreme thermophile, Thermus thermophilus HB8 (1979), Agric. Biol. Chem., 43, 397-399.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.49 0.03
-
 oxaloacetate
-
 Thermus thermophilus
4.1.1.49 0.04
-
 ADP
-
 Thermus thermophilus
4.1.1.49 0.04
-
 phosphoenolpyruvate
-
 Thermus thermophilus
4.1.1.49 0.08
-
 ATP
-
 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.49 ATP + oxaloacetate Thermus thermophilus the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway ?
-
 ?
4.1.1.49 ATP + oxaloacetate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Thermus thermophilus
-
-
-
4.1.1.49 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.49
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Thermus thermophilus ATP + oxaloacetate
-
 ?
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ATP + oxaloacetate
-
 ?
4.1.1.49 ATP + oxaloacetate
-
 Thermus thermophilus ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ATP + oxaloacetate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ATP + oxaloacetate the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway Thermus thermophilus ?
-
 ?
4.1.1.49 ATP + oxaloacetate the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.49 80
-
 50 min, stable Thermus thermophilus
4.1.1.49 90
-
 about 50% loss of activity after 20 min, about 90% loss of activity after 45 min Thermus thermophilus
4.1.1.49 97
-
 complete inactivation after 5 min Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.49 6.5
-
 phosphoenolpyruvate formation, imidazole-HCl buffer Thermus thermophilus
4.1.1.49 7.5
-
 phosphoenolpyruvate formation, Tris-HCl buffer Thermus thermophilus