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Literature summary extracted from

  • Anderson, J.J.; Dagley, S.
    Catabolism of tryptophan, anthranilate, and 2,3-dihydroxybenzoate in Trichosporon cutaneum (1981), J. Bacteriol., 146, 291-297.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.35 L-tryptophan induces Cutaneotrichosporon cutaneum
1.14.13.35 salicylate induces Cutaneotrichosporon cutaneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.46 0.037
-
 2,3-Dihydroxybenzoate
-
 Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.46 36500
-
 2 * 36500, SDS-PAGE Cutaneotrichosporon cutaneum
4.1.1.46 66100
-
 gel filtration Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.35 anthranilate + NADPH + O2 Cutaneotrichosporon cutaneum enzyme in degradation of L-tryptophan 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
4.1.1.46 2,3-Dihydroxybenzoate Cutaneotrichosporon cutaneum enzyme is involved in the catabolism of Trp, induced by both L-Trp and salicylate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.35 Cutaneotrichosporon cutaneum
-
 yeast
-
4.1.1.46 Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.35 partial, using heat treatment, DEAE-cellulose column chromatography and elution with a linear gradient of 0 to 0.1 M KCl in phosphate buffer Cutaneotrichosporon cutaneum
4.1.1.46
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.35 0.041
-
 extracts of tryptophan-grown cells Cutaneotrichosporon cutaneum
1.14.13.35 1.02
-
 partially purified enzyme Cutaneotrichosporon cutaneum
4.1.1.46 14.5
-
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.35 anthranilate + NADPH + O2
-
 Cutaneotrichosporon cutaneum 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
1.14.13.35 anthranilate + NADPH + O2 enzyme in degradation of L-tryptophan Cutaneotrichosporon cutaneum 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
4.1.1.46 2,3,5-Trihydroxybenzoate 18% of the activity with 2,3-dihydroxybenzoate Cutaneotrichosporon cutaneum 1,2,4-Trihydroxybenzene + CO2
-
 ?
4.1.1.46 2,3-Dihydroxybenzoate
-
 Cutaneotrichosporon cutaneum Catechol + CO2
-
 ?
4.1.1.46 2,3-Dihydroxybenzoate enzyme is involved in the catabolism of Trp, induced by both L-Trp and salicylate Cutaneotrichosporon cutaneum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.46 dimer 2 * 36500, SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.46 7.7
-
-
 Cutaneotrichosporon cutaneum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.46 6 9.7 about 50% of maximal activity at pH 6.0 and at pH 9.7 Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.35 NADPH absolute requirement for NADPH Cutaneotrichosporon cutaneum