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Literature summary extracted from

  • Kimura, Y.; Yasuda, N.; Tanigaki-Nagae, H.; Nakabayashi, T.; Matsunga, H.M; Kimura, M.; Matsuoka, A.
    Acetoacetate decarboxylase and a peptide with similar activity produced by Bacillus polymyxa (1986), Agric. Biol. Chem., 50, 2509-2516.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.1.1.4 analysis the enzyme is a valuable tool for clinical analysis of ketone bodies in human plasma Paenibacillus polymyxa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.4 Acetylacetone
-
 Paenibacillus polymyxa
4.1.1.4 ClO4-
-
 Paenibacillus polymyxa
4.1.1.4 HSO3-
-
 Paenibacillus polymyxa
4.1.1.4 KCN
-
 Paenibacillus polymyxa
4.1.1.4 SCN-
-
 Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.4 0.94
-
 acetoacetate
-
 Paenibacillus polymyxa
4.1.1.4 400
-
 acetoacetate polymyxin MI Paenibacillus polymyxa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.4 intracellular
-
 Paenibacillus polymyxa 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.4 27000
-
 about 10 * 27000, SDS-PAGE Paenibacillus polymyxa
4.1.1.4 280000
-
 gel filtration Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.4 Paenibacillus polymyxa
-
-
-
4.1.1.4 Paenibacillus polymyxa
-
 in addition to the intracellular enzyme, the extracellular produced polymyxin MI catalyzes the reaction
-
4.1.1.4 Paenibacillus polymyxa A-57
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.4
-
 Paenibacillus polymyxa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.4 cell culture
-
 Paenibacillus polymyxa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.4 296
-
-
 Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.4 Acetoacetate
-
 Paenibacillus polymyxa Acetone + CO2
-
 ?
4.1.1.4 Acetoacetate
-
 Paenibacillus polymyxa A-57 Acetone + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.4 ? about 10 * 27000, SDS-PAGE Paenibacillus polymyxa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.4 60
-
 30 min, stable up to Paenibacillus polymyxa
4.1.1.4 80
-
 30 min, complete inactivation Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.4 5.9
-
-
 Paenibacillus polymyxa
4.1.1.4 6
-
 polymyxin MI Paenibacillus polymyxa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.4 3 8 pH 3: about 50% of maximal activity, pH 8: about 40% of maximal activity Paenibacillus polymyxa
4.1.1.4 5 8 pH 5: about 40% of maximal activity, pH 8: about 25% of maximal activity Paenibacillus polymyxa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.4 5.5 9 4°C, 23 h, stable Paenibacillus polymyxa