BRENDA - Enzyme Database show

Ribulose-diphosphate carboxylase from spinach leaves

Siegel, M.I.; Lane, M.D.; Methods Enzymol. 42C, 472-489 (1975)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.39
(NH4)2SO4
-
Spinacia oleracea
4.1.1.39
arsenite
-
Spinacia oleracea
4.1.1.39
CN-
-
Spinacia oleracea
4.1.1.39
Hg2+
-
Spinacia oleracea
4.1.1.39
p-chloromercuribenzoate
-
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.39
0.12
-
D-ribulose 1,5-bisphosphate
-
Spinacia oleracea
4.1.1.39
22
-
CO2
-
Spinacia oleracea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.39
Cu2+
activation
Spinacia oleracea
4.1.1.39
Mg2+
activation
Spinacia oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
Spinacia oleracea
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.39
Spinacia oleracea
-
spinach
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.39
-
Spinacia oleracea
Reaction
EC Number
Reaction
Commentary
Organism
4.1.1.39
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O
D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.39
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.39
1.48
-
carboxylase
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.39
-20°C, 6-12 months
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
-
4491
Spinacia oleracea
D-3-Phosphoglycerate
-
-
-
-
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
-
4491
Spinacia oleracea
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.39
7.9
-
carboxylation
Spinacia oleracea
4.1.1.39
9.4
-
oxygenation
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.39
(NH4)2SO4
-
Spinacia oleracea
4.1.1.39
arsenite
-
Spinacia oleracea
4.1.1.39
CN-
-
Spinacia oleracea
4.1.1.39
Hg2+
-
Spinacia oleracea
4.1.1.39
p-chloromercuribenzoate
-
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.39
0.12
-
D-ribulose 1,5-bisphosphate
-
Spinacia oleracea
4.1.1.39
22
-
CO2
-
Spinacia oleracea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.39
Cu2+
activation
Spinacia oleracea
4.1.1.39
Mg2+
activation
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
Spinacia oleracea
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.39
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.39
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.39
1.48
-
carboxylase
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.39
-20°C, 6-12 months
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
-
4491
Spinacia oleracea
D-3-Phosphoglycerate
-
-
-
-
4.1.1.39
D-Ribulose 1,5-bisphosphate + CO2
-
4491
Spinacia oleracea
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.39
7.9
-
carboxylation
Spinacia oleracea
4.1.1.39
9.4
-
oxygenation
Spinacia oleracea