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Literature summary extracted from
- Ribulose-diphosphate carboxylase from spinach leaves (1975), Methods Enzymol., 42C, 472-489.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.39 | (NH4)2SO4 | - |
Spinacia oleracea |  |
| 4.1.1.39 | arsenite | - |
Spinacia oleracea | |
| 4.1.1.39 | CN- | - |
Spinacia oleracea | |
| 4.1.1.39 | Hg2+ | - |
Spinacia oleracea | |
| 4.1.1.39 | p-chloromercuribenzoate | - |
Spinacia oleracea | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.39 | 0.12 | - |
D-ribulose 1,5-bisphosphate | - |
Spinacia oleracea | |
| 4.1.1.39 | 22 | - |
CO2 | - |
Spinacia oleracea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.39 | Cu2+ | activation | Spinacia oleracea | |
| 4.1.1.39 | Mg2+ | activation | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.39 | D-Ribulose 1,5-bisphosphate + CO2 | Spinacia oleracea | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.39 | Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.39 | - |
Spinacia oleracea |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.1.39 | 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O | D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction | Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.1.39 | leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.39 | 1.48 | - |
carboxylase | Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.39 | -20°C, 6-12 months | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.39 | D-Ribulose 1,5-bisphosphate + CO2 | - |
Spinacia oleracea | D-3-Phosphoglycerate | - |
? | |
| 4.1.1.39 | D-Ribulose 1,5-bisphosphate + CO2 | - |
Spinacia oleracea | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.39 | 7.9 | - |
carboxylation | Spinacia oleracea |
| 4.1.1.39 | 9.4 | - |
oxygenation | Spinacia oleracea |
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Release 2023.1 (January 2023)
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