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Literature summary extracted from

  • Jones, R.M.; Jordan, P.M.
    Purification and properties of the uroporphyrinogen decarboxylase from Rhodobacter sphaeroides (1993), Biochem. J., 293, 703-712.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.37 dithiothreitol activates Cereibacter sphaeroides

General Stability

EC Number General Stability Organism
4.1.1.37 unstable in absence of 2-mercaptoethanol Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.37 5,5'-dithiobis(2-nitrobenzoate)
-
 Cereibacter sphaeroides
4.1.1.37 Coproporphyrin I
-
 Cereibacter sphaeroides
4.1.1.37 coproporphyrin III
-
 Cereibacter sphaeroides
4.1.1.37 Cu2+
-
 Cereibacter sphaeroides
4.1.1.37 Fe3+
-
 Cereibacter sphaeroides
4.1.1.37 Haematoporphyrin IX
-
 Cereibacter sphaeroides
4.1.1.37 Haemin
-
 Cereibacter sphaeroides
4.1.1.37 Hg2+
-
 Cereibacter sphaeroides
4.1.1.37 iodoacetamide
-
 Cereibacter sphaeroides
4.1.1.37 iodoacetate
-
 Cereibacter sphaeroides
4.1.1.37 mesoporphyrin IX
-
 Cereibacter sphaeroides
4.1.1.37 NEM
-
 Cereibacter sphaeroides
4.1.1.37 Phenylglyoxal
-
 Cereibacter sphaeroides
4.1.1.37 protoporphyrin IX
-
 Cereibacter sphaeroides
4.1.1.37 uroporphyrin III
-
 Cereibacter sphaeroides
4.1.1.37 Uroporphyrinogen I
-
 Cereibacter sphaeroides
4.1.1.37 uroporphyrinogen III
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.37 0.0018
-
 Uroporphyrinogen I
-
 Cereibacter sphaeroides
4.1.1.37 0.006
-
 uroporphyrinogen III
-
 Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.37 Mn2+ slight activation Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.37 38000 39000 gel filtration, nondenaturing PAGE Cereibacter sphaeroides
4.1.1.37 41000
-
 1 * 41000, SDS-PAGE Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.37 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.37
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.37 0.0126
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
4.1.1.37 frozen in 25 mM potassium phosphate buffer, pH 7.0, containing 2-mercaptoethanol, stable Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.37 uroporphyrinogen I
-
 Cereibacter sphaeroides coproporphyrinogen I + 4 CO2
-
 ?
4.1.1.37 Uroporphyrinogen III
-
 Cereibacter sphaeroides Coproporphyrinogen III + 4 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.37 monomer 1 * 41000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.37 55
-
 t1/2 = 8 min Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.37 6.7 6.9
-
 Cereibacter sphaeroides
4.1.1.37 6.8
-
-
 Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.37 6.2 7.7 pH 6.2: about 25% of maximal activity, pH 7.7: about 70% of maximal activity Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.37 5.5
-
 unstable below Cereibacter sphaeroides