EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.52 | expressed in Escherichia coli | Escherichia coli |
2.7.7.61 | expressed in Escherichia coli BL21-DE3 | Escherichia coli |
4.1.3.6 | apo-ACP | Klebsiella pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.52 | 32600 | - |
? * 32600, SDS-PAGE | Escherichia coli |
2.7.7.61 | 20300 | - |
x * 20300, SDS-PAGE, autoradiography | Escherichia coli |
4.1.3.6 | 11760 | - |
apo-ACP-His, mass spectrometry | Escherichia coli |
4.1.3.6 | 11760 | - |
apo-ACP-His, mass spectrometry | Klebsiella pneumoniae |
4.1.3.6 | 12500 | - |
apo-ACP-His, SDS-PAGE | Escherichia coli |
4.1.3.6 | 12500 | - |
apo-ACP-His, SDS-PAGE | Klebsiella pneumoniae |
4.1.3.6 | 12650 | - |
holo-ACP-His, mass spectrometry | Escherichia coli |
4.1.3.6 | 12650 | - |
holo-ACP-His, mass spectrometry | Klebsiella pneumoniae |
4.1.3.6 | 15000 | - |
holo-ACP-His, SDS-PAGE | Escherichia coli |
4.1.3.6 | 15000 | - |
holo-ACP-His, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.52 | ATP + 3'-dephospho-CoA | Escherichia coli | - |
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? | |
4.1.3.6 | citrate | Escherichia coli | - |
acetate + oxaloacetate | - |
? | |
4.1.3.6 | citrate | Klebsiella pneumoniae | - |
acetate + oxaloacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.52 | Escherichia coli | - |
strain JM83, strain CM2080, gene citG | - |
2.4.2.52 | Escherichia coli | P77231 | - |
- |
2.4.2.52 | Klebsiella pneumoniae | P45414 | - |
- |
2.7.7.61 | Escherichia coli | - |
- |
- |
4.1.3.6 | Escherichia coli | - |
- |
- |
4.1.3.6 | Klebsiella pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.52 | - |
Escherichia coli |
2.7.7.61 | - |
Escherichia coli |
4.1.3.6 | apo-ACP | Klebsiella pneumoniae |
4.1.3.6 | apo-ACP, i.e. gamma-subunit | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.61 | 2'-(5-triphosphoribosyl)-3'-dephospho-CoA + apo-[citrate (pro-3S)-lyase] = diphosphate + holo-[citrate (pro-3S)-lyase] | 2'-(5"-triphosphoribosyl)-3'-dephospho-CoA: prosthetic group of the gamma-subunit of the citrate lyase | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.6 | 8 | - |
- |
Klebsiella pneumoniae |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.52 | -4°C | Escherichia coli |
2.7.7.61 | 4°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.52 | ATP + 3'-dephospho-CoA | - |
Escherichia coli | 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? | |
2.4.2.52 | ATP + 3'-dephospho-CoA | ATP cannot be replaced by CTP, GTP or UTP | Escherichia coli | 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? | |
2.4.2.52 | additional information | holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA | Klebsiella pneumoniae | ? | - |
? | |
2.4.2.52 | additional information | holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA | Escherichia coli | ? | - |
? | |
2.7.7.61 | apo-ACP + ATP | side activity of the CitX in absence of its natural substrates, besides ATP, CTP, GTP, and UTP serve as nucleotidyl donors in vitro, ACP: acyl carrier protein, gamma-subunit of the citrate lyase | Escherichia coli | AMP-ACP + diphosphate | - |
? | |
2.7.7.61 | additional information | no substrate in vitro: NAD+ | Escherichia coli | ? | - |
? | |
4.1.3.6 | citrate | - |
Escherichia coli | acetate + oxaloacetate | - |
? | |
4.1.3.6 | citrate | - |
Klebsiella pneumoniae | acetate + oxaloacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.52 | ? | ? * 32600, SDS-PAGE | Escherichia coli |
2.7.7.61 | ? | x * 20300, SDS-PAGE, autoradiography | Escherichia coli |
4.1.3.6 | ? | alpha, beta, gamma | Klebsiella pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.52 | CitG | - |
Klebsiella pneumoniae |
2.4.2.52 | CitG | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.6 | 25 | - |
assay at | Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.6 | 2'-(5''-phosphoribosyl)-3'-dephospho-coenzyme A | attached to the gamma subunit, i.e. apo-ACP, via a phosphodiester linkage to serine-14, CitG and CitX catalyze the initial formation of the precursor 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA and subsequent transfer to apo-citrate lyase with removal of diphosphate | Klebsiella pneumoniae | |
4.1.3.6 | 2'-(5''-phosphoribosyl)-3'-dephospho-coenzyme A | attached to the gamma subunit, i.e. apo-ACP, via phosphodiester linkage by CitX, resulting in the formation of holo-ACP | Escherichia coli |