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Literature summary extracted from

  • Walker, K.; Croteau, R.
    Taxol biosynthesis: molecular cloning of a benzoyl-CoA:taxane 2alpha-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli (2000), Proc. Natl. Acad. Sci. USA, 97, 13591-13596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.166 expressed in Escherichia coli JM109 Taxus cuspidata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.166 0.3
-
 benzoyl-CoA
-
 Taxus cuspidata
2.3.1.166 0.64
-
 2-debenzoyl-7,13-diacetylbaccatin III
-
 Taxus cuspidata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.166 50000
-
 ca. x * 50000, SDS-PAGE Taxus cuspidata
2.3.1.166 50090
-
 calculated from sequence of cDNA Taxus cuspidata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.166 benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III Taxus cuspidata
-
 CoA + 10-deacetylbaccatin III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.166 Taxus cuspidata Q9FPW3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.166 partially Taxus cuspidata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.166 benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III
-
 Taxus cuspidata CoA + 10-deacetylbaccatin III
-
 ?
2.3.1.166 benzoyl-CoA + 2-debenzoyl-7,13-diacetylbaccatin III 2-debenzoyl-7,13-diacetylbaccatin III is a semisynthetic substrate Taxus cuspidata CoA + 7,13-diacetylbaccatin III
-
 ?
2.3.1.166 additional information enzyme does not benzoylate the 1beta-, 7beta-, 10beta- or 13alpha-hydroxyl groups of 10-deacetylbaccatin III, nor does it benzoylate the 2alpha- or 5alpha-hydroxyl groups of taxa-4(20),11(12)-dien-2alpha,5alpha-diol, acetyl-CoA no cosubstrate Taxus cuspidata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.166 ? ca. x * 50000, SDS-PAGE Taxus cuspidata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.166 31
-
 enzyme assay Taxus cuspidata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.166 8
-
-
 Taxus cuspidata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.166 6.5 9.9
-
 Taxus cuspidata