Literature summary extracted from

Auclair, K.; Sutherland, A.; Kennedy, J.; Witter, D.J.; Van den Heever, J.P.; Hutchinson, C.R.; Vederas, J.C.
Lovastatin nonaketide synthase catalyzes an intramolecular Diels-Alder reaction of a substrate analogue (2000), J. Am. Chem. Soc., 122, 11519-11520.

No PubMed abstract available

Application

EC Number	Application	Comment	Organism
2.3.1.161	medicine	production of cholesterol lowering drugs	Aspergillus terreus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.1.161	lovB and lovC protein	Aspergillus terreus

General Stability

EC Number	General Stability	Organism
2.3.1.161	inactivated by heating	Aspergillus terreus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3.1.161	0.5	-	malonyl-CoA	-	Aspergillus terreus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.3.1.161	335000	-	SDS-PAGE	Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.1.161	acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	Aspergillus terreus	reaction mechanism	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.161	Aspergillus terreus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.161	homogeneitiy	Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.1.161	0.0109	-	-	Aspergillus terreus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.1.161	acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	reaction mechanism	Aspergillus terreus	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?
2.3.1.161	acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	enzyme also displays Diels-Alderase activity in vitro	Aspergillus terreus	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?

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Release 2023.1 (January 2023)