EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.150 | expression in Escherichia coli | Medicago sativa |
2.1.1.270 | expression in Escherichia coli | Medicago sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.150 | up | IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity | Medicago sativa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.150 | 39603 | - |
x * 39603, calculated from sequence | Medicago sativa |
2.1.1.150 | 41000 | - |
x * 41000, SDS-PAGE | Medicago sativa |
2.1.1.270 | 41000 | - |
x * 41000, SDS-PAGE, recombinant enzyme | Medicago sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.150 | S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | Medicago sativa | i.e. daidzein | S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone | i.e. isoformononetin | ? | |
2.1.1.270 | additional information | Medicago sativa | in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.150 | Medicago sativa | O24529 | - |
- |
2.1.1.270 | Medicago sativa | O24529 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.270 | - |
Medicago sativa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.150 | callus | - |
Medicago sativa | - |
2.1.1.150 | cell suspension culture | - |
Medicago sativa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.150 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone | i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
2.1.1.150 | S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone | 136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
2.1.1.150 | S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein | Medicago sativa | S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone | i.e. isoformononetin | ? | |
2.1.1.270 | additional information | in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation | Medicago sativa | ? | - |
? | |
2.1.1.270 | S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain | at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 | Medicago sativa | S-adenosyl-L-homocysteine + (+)-pisatin | - |
? | |
2.1.1.270 | S-adenosyl-L-methionine + (+)-maackiain | at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 | Medicago sativa | S-adenosyl-L-homocysteine + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.150 | ? | x * 41000, SDS-PAGE | Medicago sativa |
2.1.1.150 | ? | x * 39603, calculated from sequence | Medicago sativa |
2.1.1.270 | ? | x * 41000, SDS-PAGE, recombinant enzyme | Medicago sativa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.150 | 7-IOMT | - |
Medicago sativa |
2.1.1.150 | IOMT8 | - |
Medicago sativa |
2.1.1.270 | IOMT8 | - |
Medicago sativa |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.1.1.270 | Medicago sativa | IOMT8 transcripts are rapidly induced following elicitation | up |