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Literature summary extracted from

  • Liu, J.Q.; Ito, S.; Dairi, T.; Itoh, N.; Kataoka, M.; Shimizu, S.; Yamada, H.
    Gene cloning, nucleotide sequencing, and purification and characterization of the low-specificity L-threonine aldolase from Pseudomonas sp. strain NCIMB 10558 (1998), Appl. Environ. Microbiol., 64, 549-554.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.48 overexpression in Escherichia coli Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.48 K207A the mutant enzyme shows no detectable enzyme activity. The mutant enzyme show the disappearance of the absorption maximum at 420 nm, indicating that the Schiff base linkage between the epsilon-amino group of the active-site lysine residue and the pyridoxal 5'-phosphate cofactor aldehyde group of the wild type is not present in the mutant enzyme Pseudomonas sp.
4.1.2.48 K207R the mutant enzyme shows a specific activity of about 1000 times lower than that of the wild-type enzyme. The mutant enzyme show the disappearance of the absorption maximum at 420 nm, indicating that the Schiff base linkage between the epsilon-amino group of the active-site lysine residue and the pyridoxal 5'-phosphate cofactor aldehyde group of the wild type is not present in the mutant enzyme Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.48 7.3
-
 L-threo-phenylserine pH 8.0, 30°C Pseudomonas sp.
4.1.2.48 7.4
-
 L-threo-beta-3,4-methylenedioxyphenylserine pH 8.0, 30°C Pseudomonas sp.
4.1.2.48 8.3
-
 L-threo-beta-3,4-dihydroxyphenylserine pH 8.0, 30°C Pseudomonas sp.
4.1.2.48 10.2
-
 L-erythro-phenylserine pH 8.0, 30°C Pseudomonas sp.
4.1.2.48 14.6
-
 L-allo-threonine pH 8.0, 30°C Pseudomonas sp.
4.1.2.48 14.7
-
 L-threonine pH 8.0, 30°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.48 38000
-
 4 * 38000, SDS-PAGE Pseudomonas sp.
4.1.2.48 145000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Escherichia coli catalyzes interconversion of serine and glycine glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Escherichia coli
-
-
-
4.1.2.48 Pseudomonas sp. O50584
-
-
4.1.2.48 Pseudomonas sp. NCIMB 10558 O50584
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.48
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.48 41
-
 pH 8.0, 30°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Escherichia coli glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate catalyzes interconversion of serine and glycine Escherichia coli glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
4.1.2.48 L-allo-threonine
-
 Pseudomonas sp. glycine + acetaldehyde
-
 r
4.1.2.48 L-allo-threonine
-
 Pseudomonas sp. NCIMB 10558 glycine + acetaldehyde
-
 r
4.1.2.48 L-erythro-phenylserine
-
 Pseudomonas sp. glycine + benzaldehyde
-
 ?
4.1.2.48 L-erythro-phenylserine
-
 Pseudomonas sp. NCIMB 10558 glycine + benzaldehyde
-
 ?
4.1.2.48 L-threo-beta-3,4-dihydroxyphenylserine
-
 Pseudomonas sp. glycine + 3,4-dihydroxybenzaldehyde
-
 ?
4.1.2.48 L-threo-beta-3,4-dihydroxyphenylserine
-
 Pseudomonas sp. NCIMB 10558 glycine + 3,4-dihydroxybenzaldehyde
-
 ?
4.1.2.48 L-threo-beta-3,4-methylenedioxyphenylserine
-
 Pseudomonas sp. ?
-
 ?
4.1.2.48 L-threo-beta-3,4-methylenedioxyphenylserine
-
 Pseudomonas sp. NCIMB 10558 ?
-
 ?
4.1.2.48 L-threo-phenylserine
-
 Pseudomonas sp. glycine + benzaldehyde
-
 ?
4.1.2.48 L-threonine
-
 Pseudomonas sp. glycine + acetaldehyde
-
 r
4.1.2.48 L-threonine
-
 Pseudomonas sp. NCIMB 10558 glycine + acetaldehyde
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.48 tetramer 4 * 38000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.2.48 L-TA
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.48 25
-
-
 Pseudomonas sp.
4.1.2.48 30
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.48 25
-
 40°C, 15 min, 50% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.48 8
-
 assay at Pseudomonas sp.
4.1.2.48 8 8.5
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.48 5.5 9 30°C, 30 min, stable Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.48 pyridoxal 5'-phosphate contains 1 mol of pyridoxal 5'-phosphate per mol of 38000 da subunit. Lys207 probably functions as an essential catalytic residue, forming an internal Schiff base with the pyridoxal 5'-phosphate of the enzyme to catalyze the reversible aldol reaction Pseudomonas sp.