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Literature summary extracted from

  • Trivedi, V.; Gupta, A.; Jala, V.R.; Saravanan, P.; Rao, G.S.J.; Rao, N.A.; Savithri, H.S.; Subramanya, H.S.
    Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism (2002), J. Biol. Chem., 277, 17161-17169.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.1 medicine enzyme is a potential target for cancer chemotherapy Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 internal aldimine form, external aldimine form with bound serine and glycine, ternary complex with glycine and 5-formyl tetrahydrofolate Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Geobacillus stearothermophilus
-
 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Geobacillus stearothermophilus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine evidence for a direct transfer mechanism for the enzyme catalysed reaction Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 additional information enzyme catalyses the racemization of D- and L-alanine Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer gel filtration, crystallographic symmetry Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus