Literature summary extracted from

Kruschwitz, H.; Ren, S.; Di Salvo, M.; Schifch, V.
Expression, purification, and characterization of human cytosolic serine hydroxymethyltransferase (1995), Protein Expr. Purif., 6, 411-416.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.1.2.1	expression in Escherichia coli	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1.2.1	0.04	0.046	tetrahydrofolate	recombinant enzyme	Homo sapiens
2.1.2.1	0.15	0.9	L-serine	recombinant enzyme	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.1.2.1	cytoplasm	-	Homo sapiens	5737	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.2.1	Homo sapiens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.1.2.1	recombinant enzyme	Homo sapiens

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.1.2.1	10	-	recombinant enzyme	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.2.1	L-serine + tetrahydrofolate	-	Homo sapiens	glycine + 5,10-methylenetetrahydrofolate + H2O	-	r

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1.2.1	9.58	-	L-serine	-	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.1.2.1	tetrahydrofolate	requirement with L-serine or L-2-methylserine as substrate	Homo sapiens

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Release 2023.1 (January 2023)