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Literature summary extracted from

  • Ramesh, K.S.; Rao, N.A.
    Purification and physicochemical, kinetic and immunological properties of allosteric serine hydroxymethyltransferase from monkey liver (1980), Biochem. J., 187, 623-636.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.1 additional information in the presence of 1mM dithiothreitol + 1mM pyridoxal 5-phosphate + 20 mM EDTA: 29fold increase in the specific activity Macaca radiata

General Stability

EC Number General Stability Organism
2.1.2.1 2-mercaptoethanol stabilizes Macaca radiata
2.1.2.1 DTT stabilizes Macaca radiata
2.1.2.1 EDTA stabilizes Macaca radiata
2.1.2.1 pyridoxal 5'-phosphate stabilizes Macaca radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 aminopterin
-
 Macaca radiata
2.1.2.1 D-cycloserine
-
 Macaca radiata
2.1.2.1 Dichloromethotrexate
-
 Macaca radiata
2.1.2.1 dihydrofolate
-
 Macaca radiata
2.1.2.1 glycine
-
 Macaca radiata
2.1.2.1 methotrexate
-
 Macaca radiata
2.1.2.1 additional information inhibition kinetics Macaca radiata
2.1.2.1 tetrahydrofolate derivatives
-
 Macaca radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information allosteric kinetics Macaca radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm 75% of the enzyme activity Macaca radiata 5737
-
2.1.2.1 microsome 3% of the enzyme activity Macaca radiata
-
-
2.1.2.1 mitochondrion 5% of the enzyme activity Macaca radiata 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 52000
-
 4 * 52000, SDS-PAGE Macaca radiata
2.1.2.1 208000
-
 gel filtration Macaca radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Macaca radiata regulatory protein glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Macaca radiata
-
 Bonnet monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 heat-denaturation omitted Macaca radiata

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Macaca radiata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 brain
-
 Macaca radiata
-
2.1.2.1 kidney
-
 Macaca radiata
-
2.1.2.1 additional information distribution Macaca radiata
-
2.1.2.1 pancreas
-
 Macaca radiata
-
2.1.2.1 skeletal muscle
-
 Macaca radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Macaca radiata
2.1.2.1 3.4
-
-
 Macaca radiata

Storage Stability

EC Number Storage Stability Organism
2.1.2.1 -20°C, 6-8 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA Macaca radiata
2.1.2.1 0°C, at least 2 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA Macaca radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Macaca radiata glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate regulatory protein Macaca radiata glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 additional information insoluble enzyme/antibody-complex shows 90% of original activity Macaca radiata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homotetramer 4 * 52000, SDS-PAGE Macaca radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 60
-
-
 Macaca radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 additional information
-
 L-serine protects against thermal inactivation Macaca radiata
2.1.2.1 additional information
-
 enzyme-antibody complex more stable to elevated temperatures than free enzyme, allosteric effectors fail to protect free enzyme Macaca radiata
2.1.2.1 50
-
 20 min stable Macaca radiata
2.1.2.1 65
-
 85% loss of activity after 20 min Macaca radiata
2.1.2.1 70
-
 complete loss of activity after 6 min, enzyme-antibody complex: 30% loss of activity after 20 min Macaca radiata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Macaca radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 additional information
-
 pI: 4.2 Macaca radiata
2.1.2.1 7.4
-
-
 Macaca radiata

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate requirement, 4 mol per mol enzyme Macaca radiata
2.1.2.1 tetrahydrofolate
-
 Macaca radiata